Media and materials

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Katherine Groff. Increasing the use of sequence-defined antibodies: Practical solutions for common targets. ALTEX - Alternatives to Animal Experimentation. 2026;43(2):347-352. doi:10.14573/altex.2512091
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Shaianne N. Stein, Lorena R. Braid. An animal component-free bioprocess for synthesizing 3D human matrix scaffolds using mesenchymal stromal cells. Frontiers in Cell and Developmental Biology. 2026;14. doi:10.3389/fcell.2026.1624745
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Zahra Miri, Johanna Laakkonen, Emilia Toivonen, Niina Väljä, Susanna Miettinen, Hanna Vuorenpää. Establishment of human-relevant in vitro models using animal-free serum replacement and recombinant antibodies. Frontiers in Toxicology. 2026;8. doi:10.3389/ftox.2026.1741716
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Huiyuan Meng, Philip J. R. Day. Exploring ethical, sustainable and effective foetal bovine serum alternatives for in vitro mammalian cell culture. Frontiers in Toxicology. 2026;8. doi:10.3389/ftox.2026.1776815
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Tilo Weber, Atena Malakpour-Permlid, Aline Chary, et al. Fetal bovine serum: how to leave it behind in the pursuit of more reliable science. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1612903
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Esther J. M. Liefting, Jeffrey J. Bajramovic. Optimizing Wnt activation in fetal calf serum (FCS)-free organoid expansion media. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1504469
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Shamili Immalaraju, Srishti Goyal, Rukmini Jonnalagadda. Towards the standardization of human platelet lysate production and its comparison to fetal bovine serum for human hematopoietic cell culture: a scoping review. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1496231
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Atena Malakpour-Permlid, Manuel Marcos Rodriguez, Kinga Zór, Anja Boisen, Stina Oredsson. Advancing humanized 3D tumor modeling using an open access xeno-free medium. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1529360
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Alastair G. Stewart, Avanka Gunatilaka, Bryan Gao. Should culture media for microphysiological systems be nutritionally physiological? Nature Reviews Bioengineering. 2025;3(5):357-358. doi:10.1038/s44222-025-00290-w
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Wim B. M. de Lau, Joost J. A. P. M. Wijnakker, Gijs J. F. van Son, et al. A single-chain derivative of an integrin-activating antibody potentiates organoid growth in Matrigel and collagen hydrogels. Nature Biotechnology. 2025:1-11. doi:10.1038/s41587-025-02874-8
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