%0 Journal Article %K alternatives to animal testing %K In vitro skin irritation testing %K Subcutis %K Tissue engineering %K three-layered skin model %B Frontiers in Bioengineering and Biotechnology %D 2020 %G English %R 10.3389/fbioe.2020.00388 %T Improvement of a Three-Layered in vitro Skin Model for Topical Application of Irritating Substances %U https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2020.00388/full %V 8 %8 2020-05-08