TY - JOUR
KW - Antimicrobial responses
KW - Innate immunity
KW - Tissue engineering
KW - Viral infection
AU - Haiqing Bai
AU - Longlong Si
AU - Amanda Jiang
AU - Chaitra Belgur
AU - Yunhao Zhai
AU - Roberto Plebani
AU - Crystal Yuri Oh
AU - Melissa Rodas
AU - Aditya Patil
AU - Atiq Nurani
AU - Sarah E. Gilpin
AU - Rani K. Powers
AU - Girija Goyal
AU - Rachelle Prantil-Baun
AU - Donald E. Ingber
AB - Mechanical breathing motions have a fundamental function in lung development and disease, but little is known about how they contribute to host innate immunity. Here we use a human lung alveolus chip that experiences cyclic breathing-like deformations to investigate whether physical forces influence innate immune responses to viral infection. Influenza H3N2 infection of mechanically active chips induces a cascade of host responses including increased lung permeability, apoptosis, cell regeneration, cytokines production, and recruitment of circulating immune cells. Comparison with static chips reveals that breathing motions suppress viral replication by activating protective innate immune responses in epithelial and endothelial cells, which are mediated in part through activation of the mechanosensitive ion channel TRPV4 and signaling via receptor for advanced glycation end products (RAGE). RAGE inhibitors suppress cytokines induction, while TRPV4 inhibition attenuates both inflammation and viral burden, in infected chips with breathing motions. Therefore, TRPV4 and RAGE may serve as new targets for therapeutic intervention in patients infected with influenza and other potential pandemic viruses that cause life-threatening lung inflammation.
BT - Nature Communications
DA - 2022-04-08
DO - 10.1038/s41467-022-29562-4
IS - 1
LA - en
N2 - Mechanical breathing motions have a fundamental function in lung development and disease, but little is known about how they contribute to host innate immunity. Here we use a human lung alveolus chip that experiences cyclic breathing-like deformations to investigate whether physical forces influence innate immune responses to viral infection. Influenza H3N2 infection of mechanically active chips induces a cascade of host responses including increased lung permeability, apoptosis, cell regeneration, cytokines production, and recruitment of circulating immune cells. Comparison with static chips reveals that breathing motions suppress viral replication by activating protective innate immune responses in epithelial and endothelial cells, which are mediated in part through activation of the mechanosensitive ion channel TRPV4 and signaling via receptor for advanced glycation end products (RAGE). RAGE inhibitors suppress cytokines induction, while TRPV4 inhibition attenuates both inflammation and viral burden, in infected chips with breathing motions. Therefore, TRPV4 and RAGE may serve as new targets for therapeutic intervention in patients infected with influenza and other potential pandemic viruses that cause life-threatening lung inflammation.
PY - 2022
EP - 1928
T2 - Nature Communications
TI - Mechanical control of innate immune responses against viral infection revealed in a human lung alveolus chip
UR - https://www.nature.com/articles/s41467-022-29562-4
VL - 13
Y2 - 2022-06-02
SN - 2041-1723
ER -