TY - JOUR KW - Animals KW - Female KW - Hormones KW - Humans KW - Hydrogels KW - Ovariectomy KW - Ovary KW - Rats KW - Spheroids, Cellular AU - Hyo-Jin Yoon AU - Yong Jae Lee AU - Sewoom Baek AU - Young Shin Chung AU - Dae-Hyun Kim AU - Jae Hoon Lee AU - Yong Cheol Shin AU - Young Min Shin AU - Chungsoon Ryu AU - Hye-Seon Kim AU - So Hyun Ahn AU - Heeyon Kim AU - Young Bin Won AU - Inha Lee AU - Myung Jae Jeon AU - Si Hyun Cho AU - Byung Seok Lee AU - Hak-Joon Sung AU - Young Sik Choi AB - The regeneration potential of implantable organ model hydrogels is applied to treat a loss of ovarian endocrine function in women experiencing menopause and/or cancer therapy. A rat ovariectomy model is used to harvest autologous ovary cells while subsequently producing a layer-by-layer form of follicle spheroids. Implantation of a microchannel network hydrogel with cell spheroids [vascularized hydrogel with ovarian spheroids (VHOS)] into an ischemic hindlimb of ovariectomized rats significantly aids the recovery of endocrine function with hormone release, leading to full endometrium regeneration. The VHOS implantation effectively suppresses the side effects observed with synthetic hormone treatment (i.e., tissue overgrowth, hyperplasia, cancer progression, deep vein thrombosis) to the normal levels, while effectively preventing the representative aftereffects of menopause (i.e., gaining fatty weight, inducing osteoporosis). These results highlight the unprecedented therapeutic potential of an implantable VHOS against menopause and suggest that it may be used as an alternative approach to standard hormone therapy. BT - Science Advances DA - 2021-04 DO - 10.1126/sciadv.abe8873 IS - 18 LA - eng N2 - The regeneration potential of implantable organ model hydrogels is applied to treat a loss of ovarian endocrine function in women experiencing menopause and/or cancer therapy. A rat ovariectomy model is used to harvest autologous ovary cells while subsequently producing a layer-by-layer form of follicle spheroids. Implantation of a microchannel network hydrogel with cell spheroids [vascularized hydrogel with ovarian spheroids (VHOS)] into an ischemic hindlimb of ovariectomized rats significantly aids the recovery of endocrine function with hormone release, leading to full endometrium regeneration. The VHOS implantation effectively suppresses the side effects observed with synthetic hormone treatment (i.e., tissue overgrowth, hyperplasia, cancer progression, deep vein thrombosis) to the normal levels, while effectively preventing the representative aftereffects of menopause (i.e., gaining fatty weight, inducing osteoporosis). These results highlight the unprecedented therapeutic potential of an implantable VHOS against menopause and suggest that it may be used as an alternative approach to standard hormone therapy. PY - 2021 EP - eabe8873 T2 - Science Advances TI - Hormone autocrination by vascularized hydrogel delivery of ovary spheroids to rescue ovarian dysfunctions VL - 7 SN - 2375-2548 ER -