TY - JOUR
KW - Animals
KW - Female
KW - Humans
KW - Menstrual Cycle
KW - Mesothelin
KW - Mice
KW - Microfluidic Analytical Techniques
KW - Ovary
KW - Pregnancy
KW - Tissue Culture Techniques
AU - Shuo Xiao
AU - Jonathan R. Coppeta
AU - Hunter B. Rogers
AU - Brett C. Isenberg
AU - Jie Zhu
AU - Susan A. Olalekan
AU - Kelly E. McKinnon
AU - Danijela Dokic
AU - Alexandra S. Rashedi
AU - Daniel J. Haisenleder
AU - Saurabh S. Malpani
AU - Chanel A. Arnold-Murray
AU - Kuanwei Chen
AU - Mingyang Jiang
AU - Lu Bai
AU - Catherine T. Nguyen
AU - Jiyang Zhang
AU - Monica M. Laronda
AU - Thomas J. Hope
AU - Kruti P. Maniar
AU - Mary Ellen Pavone
AU - Michael J. Avram
AU - Elizabeth C. Sefton
AU - Spiro Getsios
AU - Joanna E. Burdette
AU - J. Julie Kim
AU - Jeffrey T. Borenstein
AU - Teresa K. Woodruff
AB - The endocrine system dynamically controls tissue differentiation and homeostasis, but has not been studied using dynamic tissue culture paradigms. Here we show that a microfluidic system supports murine ovarian follicles to produce the human 28-day menstrual cycle hormone profile, which controls human female reproductive tract and peripheral tissue dynamics in single, dual and multiple unit microfluidic platforms (Solo-MFP, Duet-MFP and Quintet-MPF, respectively). These systems simulate the in vivo female reproductive tract and the endocrine loops between organ modules for the ovary, fallopian tube, uterus, cervix and liver, with a sustained circulating flow between all tissues. The reproductive tract tissues and peripheral organs integrated into a microfluidic platform, termed EVATAR, represents a powerful new in vitro tool that allows organ-organ integration of hormonal signalling as a phenocopy of menstrual cycle and pregnancy-like endocrine loops and has great potential to be used in drug discovery and toxicology studies.
BT - Nature Communications
DA - 2017-03-28
DO - 10.1038/ncomms14584
LA - eng
N2 - The endocrine system dynamically controls tissue differentiation and homeostasis, but has not been studied using dynamic tissue culture paradigms. Here we show that a microfluidic system supports murine ovarian follicles to produce the human 28-day menstrual cycle hormone profile, which controls human female reproductive tract and peripheral tissue dynamics in single, dual and multiple unit microfluidic platforms (Solo-MFP, Duet-MFP and Quintet-MPF, respectively). These systems simulate the in vivo female reproductive tract and the endocrine loops between organ modules for the ovary, fallopian tube, uterus, cervix and liver, with a sustained circulating flow between all tissues. The reproductive tract tissues and peripheral organs integrated into a microfluidic platform, termed EVATAR, represents a powerful new in vitro tool that allows organ-organ integration of hormonal signalling as a phenocopy of menstrual cycle and pregnancy-like endocrine loops and has great potential to be used in drug discovery and toxicology studies.
PY - 2017
EP - 14584
T2 - Nature Communications
TI - A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle
VL - 8
SN - 2041-1723
ER -