TY - JOUR
KW - Biomedical Engineering
KW - Experimental models of disease
KW - Lab-on-a-chip
KW - microfluidics
KW - Tissue engineering
AU - Zohreh Izadifar
AU - Justin Cotton
AU - Siyu Chen
AU - Viktor Horvath
AU - Anna Stejskalova
AU - Aakanksha Gulati
AU - Nina T. LoGrande
AU - Bogdan Budnik
AU - Sanjid Shahriar
AU - Erin R. Doherty
AU - Yixuan Xie
AU - Tania To
AU - Sarah E. Gilpin
AU - Adama M. Sesay
AU - Girija Goyal
AU - Carlito B. Lebrilla
AU - Donald E. Ingber
AB - Modulation of the cervix by steroid hormones and commensal microbiome play a central role in the health of the female reproductive tract. Here we describe organ-on-a-chip (Organ Chip) models that recreate the human cervical epithelial-stromal interface with a functional epithelial barrier and production of mucus with biochemical and hormone-responsive properties similar to living cervix. When Cervix Chips are populated with optimal healthy versus dysbiotic microbial communities (dominated by Lactobacillus crispatus and Gardnerella vaginalis, respectively), significant differences in tissue innate immune responses, barrier function, cell viability, proteome, and mucus composition are observed that are similar to those seen in vivo. Thus, human Cervix Organ Chips represent physiologically relevant in vitro models to study cervix physiology and host-microbiome interactions, and hence may be used as a preclinical testbed for development of therapeutic interventions to enhance women’s health.
BT - Nature Communications
DA - 2024-05-29
DO - 10.1038/s41467-024-48910-0
IS - 1
LA - en
N2 - Modulation of the cervix by steroid hormones and commensal microbiome play a central role in the health of the female reproductive tract. Here we describe organ-on-a-chip (Organ Chip) models that recreate the human cervical epithelial-stromal interface with a functional epithelial barrier and production of mucus with biochemical and hormone-responsive properties similar to living cervix. When Cervix Chips are populated with optimal healthy versus dysbiotic microbial communities (dominated by Lactobacillus crispatus and Gardnerella vaginalis, respectively), significant differences in tissue innate immune responses, barrier function, cell viability, proteome, and mucus composition are observed that are similar to those seen in vivo. Thus, human Cervix Organ Chips represent physiologically relevant in vitro models to study cervix physiology and host-microbiome interactions, and hence may be used as a preclinical testbed for development of therapeutic interventions to enhance women’s health.
PY - 2024
EP - 4578
T2 - Nature Communications
TI - Mucus production, host-microbiome interactions, hormone sensitivity, and innate immune responses modeled in human cervix chips
UR - https://www.nature.com/articles/s41467-024-48910-0
VL - 15
Y2 - 2024-11-26
SN - 2041-1723
ER -