TY - JOUR
KW - RNA sequencing
KW - Transcriptomics
AU - Magda Marečková
AU - Luz Garcia-Alonso
AU - Marie Moullet
AU - Valentina Lorenzi
AU - Robert Petryszak
AU - Carmen Sancho-Serra
AU - Agnes Oszlanczi
AU - Cecilia Icoresi Mazzeo
AU - Frederick C. K. Wong
AU - Iva Kelava
AU - Sophie Hoffman
AU - Michał Krassowski
AU - Kurtis Garbutt
AU - Kezia Gaitskell
AU - Slaveya Yancheva
AU - Ee Von Woon
AU - Victoria Male
AU - Ingrid Granne
AU - Karin Hellner
AU - Krishnaa T. Mahbubani
AU - Kourosh Saeb-Parsy
AU - Mohammad Lotfollahi
AU - Elena Prigmore
AU - Jennifer Southcombe
AU - Rebecca A. Dragovic
AU - Christian M. Becker
AU - Krina T. Zondervan
AU - Roser Vento-Tormo
AB - The complex and dynamic cellular composition of the human endometrium remains poorly understood. Previous endometrial single-cell atlases profiled few donors and lacked consensus in defining cell types. We introduce the Human Endometrial Cell Atlas (HECA), a high-resolution single-cell reference atlas (313,527 cells) combining published and new endometrial single-cell transcriptomics datasets of 63 women with and without endometriosis. HECA assigns consensus and identifies previously unreported cell types, mapped in situ using spatial transcriptomics and validated using a new independent single-nuclei dataset (312,246 nuclei, 63 donors). In the functionalis, we identify intricate stromal–epithelial cell coordination via transforming growth factor beta (TGFβ) signaling. In the basalis, we define signaling between fibroblasts and an epithelial population expressing progenitor markers. Integration of HECA with large-scale endometriosis genome-wide association study data pinpoints decidualized stromal cells and macrophages as most likely dysregulated in endometriosis. The HECA is a valuable resource for studying endometrial physiology and disorders, and for guiding microphysiological in vitro systems development.
BT - Nature Genetics
DA - 2024-09
DO - 10.1038/s41588-024-01873-w
IS - 9
LA - en
N2 - The complex and dynamic cellular composition of the human endometrium remains poorly understood. Previous endometrial single-cell atlases profiled few donors and lacked consensus in defining cell types. We introduce the Human Endometrial Cell Atlas (HECA), a high-resolution single-cell reference atlas (313,527 cells) combining published and new endometrial single-cell transcriptomics datasets of 63 women with and without endometriosis. HECA assigns consensus and identifies previously unreported cell types, mapped in situ using spatial transcriptomics and validated using a new independent single-nuclei dataset (312,246 nuclei, 63 donors). In the functionalis, we identify intricate stromal–epithelial cell coordination via transforming growth factor beta (TGFβ) signaling. In the basalis, we define signaling between fibroblasts and an epithelial population expressing progenitor markers. Integration of HECA with large-scale endometriosis genome-wide association study data pinpoints decidualized stromal cells and macrophages as most likely dysregulated in endometriosis. The HECA is a valuable resource for studying endometrial physiology and disorders, and for guiding microphysiological in vitro systems development.
PY - 2024
SP - 1925
EP - 1937
T2 - Nature Genetics
TI - An integrated single-cell reference atlas of the human endometrium
UR - https://www.nature.com/articles/s41588-024-01873-w
VL - 56
Y2 - 2024-11-26
SN - 1546-1718
ER -