TY - JOUR
KW - Adult Stem Cells
KW - Cell biology
AU - Alessandro Cherubini
AU - Francesco Rusconi
AU - Roberta Piras
AU - Kaja Nicole Wächtershäuser
AU - Marta Dossena
AU - Mario Barilani
AU - Cecilia Mei
AU - Lotta Hof
AU - Valeria Sordi
AU - Francesco Pampaloni
AU - Vincenza Dolo
AU - Lorenzo Piemonti
AU - Lorenza Lazzari
AB - Human organoids have been proposed to be powerful tools mimicking the physiopathological processes of the organs of origin. Recently, human pancreatic organoids (hPOs) have gained increasing attention due to potential theragnostic and regenerative medicine applications. However, the cellular components of hPOs have not been defined precisely. In this work, we finely characterized these structures, focusing first on morphology and identity-defining molecular features under long-term culture conditions. Next, we focused our attention on hPOs cell type composition using single-cell RNA sequencing founding a complex heterogeneity in ductal components, ranging from progenitor components to terminally differentiated ducts. Furthermore, an extensive comparison of human pancreatic organoids with previously reported transcriptomics signature of human and mouse pancreatic ductal populations, confirmed the functional pancreatic duct subpopulation heterogeneity. Finally, we showed that pancreatic organoid cells follow a precise developmental trajectory and utilize diverse signalling mechanisms, including EGF and SPP1, to facilitate cell-cell communication and maturation. Together our results offer an in-depth description of human pancreatic organoids providing a strong foundation for future in vitro diagnostic and translational studies of pancreatic health and disease.
BT - Communications Biology
DA - 2024-11-18
DO - 10.1038/s42003-024-07193-3
IS - 1
LA - en
N2 - Human organoids have been proposed to be powerful tools mimicking the physiopathological processes of the organs of origin. Recently, human pancreatic organoids (hPOs) have gained increasing attention due to potential theragnostic and regenerative medicine applications. However, the cellular components of hPOs have not been defined precisely. In this work, we finely characterized these structures, focusing first on morphology and identity-defining molecular features under long-term culture conditions. Next, we focused our attention on hPOs cell type composition using single-cell RNA sequencing founding a complex heterogeneity in ductal components, ranging from progenitor components to terminally differentiated ducts. Furthermore, an extensive comparison of human pancreatic organoids with previously reported transcriptomics signature of human and mouse pancreatic ductal populations, confirmed the functional pancreatic duct subpopulation heterogeneity. Finally, we showed that pancreatic organoid cells follow a precise developmental trajectory and utilize diverse signalling mechanisms, including EGF and SPP1, to facilitate cell-cell communication and maturation. Together our results offer an in-depth description of human pancreatic organoids providing a strong foundation for future in vitro diagnostic and translational studies of pancreatic health and disease.
PY - 2024
SP - 1
EP - 16
T2 - Communications Biology
TI - Exploring human pancreatic organoid modelling through single-cell RNA sequencing analysis
UR - https://www.nature.com/articles/s42003-024-07193-3
VL - 7
Y2 - 2024-11-22
SN - 2399-3642
ER -