TY - JOUR
KW - Blood Vessels
KW - cardiac tissues
KW - coaxial bioprinting
KW - granular hydrogels
KW - vasculature
AU - Paul P. Stankey
AU - Katharina T. Kroll
AU - Alexander J. Ainscough
AU - Daniel S. Reynolds
AU - Alexander Elamine
AU - Ben T. Fichtenkort
AU - Sebastien G.M. Uzel
AU - Jennifer A. Lewis
AB - Printing human tissues and organs replete with biomimetic vascular networks is of growing interest. While it is possible to embed perfusable channels within acellular and densely cellular matrices, they do not currently possess the biomimetic architectures found in native vessels. Here, coaxial sacrificial writing into functional tissues (co-SWIFT) is developed, an embedded bioprinting method capable of generating hierarchically branching, multilayered vascular networks within both granular hydrogel and densely cellular matrices. Coaxial printheads are designed with an extended core–shell configuration to facilitate robust core–core and shell–shell interconnections between printed branching vessels during embedded bioprinting. Using optimized core–shell ink combinations, biomimetic vessels composed of a smooth muscle cell-laden shell that surrounds perfusable lumens are coaxially printed into granular matrices composed of: 1) transparent alginate microparticles, 2) sacrificial microparticle-laden collagen, or 3) cardiac spheroids derived from human induced pluripotent stem cells. Biomimetic blood vessels that exhibit good barrier function are produced by seeding these interconnected lumens with a confluent layer of endothelial cells. Importantly, it is found that co-SWIFT cardiac tissues mature under perfusion, beat synchronously, and exhibit a cardio-effective drug response in vitro. This advance opens new avenues for the scalable biomanufacturing of vascularized organ-specific tissues for drug testing, disease modeling, and therapeutic use.
BT - Advanced Materials
DA - 2024-08-02
DO - 10.1002/adma.202401528
LA - en
N2 - Printing human tissues and organs replete with biomimetic vascular networks is of growing interest. While it is possible to embed perfusable channels within acellular and densely cellular matrices, they do not currently possess the biomimetic architectures found in native vessels. Here, coaxial sacrificial writing into functional tissues (co-SWIFT) is developed, an embedded bioprinting method capable of generating hierarchically branching, multilayered vascular networks within both granular hydrogel and densely cellular matrices. Coaxial printheads are designed with an extended core–shell configuration to facilitate robust core–core and shell–shell interconnections between printed branching vessels during embedded bioprinting. Using optimized core–shell ink combinations, biomimetic vessels composed of a smooth muscle cell-laden shell that surrounds perfusable lumens are coaxially printed into granular matrices composed of: 1) transparent alginate microparticles, 2) sacrificial microparticle-laden collagen, or 3) cardiac spheroids derived from human induced pluripotent stem cells. Biomimetic blood vessels that exhibit good barrier function are produced by seeding these interconnected lumens with a confluent layer of endothelial cells. Importantly, it is found that co-SWIFT cardiac tissues mature under perfusion, beat synchronously, and exhibit a cardio-effective drug response in vitro. This advance opens new avenues for the scalable biomanufacturing of vascularized organ-specific tissues for drug testing, disease modeling, and therapeutic use.
PY - 2024
T2 - Advanced Materials
TI - Embedding Biomimetic Vascular Networks via Coaxial Sacrificial Writing into Functional Tissue
UR - https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.202401528
Y2 - 2024-08-13
SN - 1521-4095
ER -