TY - JOUR
AU - Caitlin Lynch
AU - Srilatha Sakamuru
AU - Masato Ooka
AU - Ruili Huang
AU - Carleen Klumpp-Thomas
AU - Paul Shinn
AU - David Gerhold
AU - Anna Rossoshek
AU - Sam Michael
AU - Warren Casey
AU - Michael F. Santillo
AU - Suzanne Fitzpatrick
AU - Russell S. Thomas
AU - Anton Simeonov
AU - Menghang Xia
AB - Traditionally, chemical toxicity is determined by in vivo animal studies, which are low throughput, expensive, and sometimes fail to predict compound toxicity in humans. Due to the increasing number of chemicals in use and the high rate of drug candidate failure due to toxicity, it is imperative to develop in vitro, high-throughput screening methods to determine toxicity. The Tox21 program, a unique research consortium of federal public health agencies, was established to address and identify toxicity concerns in a high-throughput, concentration-responsive manner using a battery of in vitro assays. In this article, we review the advancements in high-throughput robotic screening methodology and informatics processes to enable the generation of toxicological data, and their impact on the field; further, we discuss the future of assessing environmental toxicity utilizing efficient and scalable methods that better represent the corresponding biological and toxicodynamic processes in humans.
BT - Annual Review of Pharmacology and Toxicology
DA - 2024/01/23
DO - 10.1146/annurev-pharmtox-112122-104310
IS - Volume 64, 2024
LA - en
N2 - Traditionally, chemical toxicity is determined by in vivo animal studies, which are low throughput, expensive, and sometimes fail to predict compound toxicity in humans. Due to the increasing number of chemicals in use and the high rate of drug candidate failure due to toxicity, it is imperative to develop in vitro, high-throughput screening methods to determine toxicity. The Tox21 program, a unique research consortium of federal public health agencies, was established to address and identify toxicity concerns in a high-throughput, concentration-responsive manner using a battery of in vitro assays. In this article, we review the advancements in high-throughput robotic screening methodology and informatics processes to enable the generation of toxicological data, and their impact on the field; further, we discuss the future of assessing environmental toxicity utilizing efficient and scalable methods that better represent the corresponding biological and toxicodynamic processes in humans.
PY - 2024
SP - 191
EP - 209
ST - High-Throughput Screening to Advance In Vitro Toxicology
T2 - Annual Review of Pharmacology and Toxicology
TI - High-Throughput Screening to Advance In Vitro Toxicology: Accomplishments, Challenges, and Future Directions
UR - https://www.annualreviews.org/content/journals/10.1146/annurev-pharmtox-112122-104310
VL - 64
Y2 - 2024-07-30
SN - 0362-1642, 1545-4304
ER -