TY - JOUR
KW - Biomedical Engineering
KW - Organogenesis
KW - Skin models
AU - Hasan Erbil Abaci
AU - Abigail Coffman
AU - Yanne Doucet
AU - James Chen
AU - Joanna Jacków
AU - Etienne Wang
AU - Zongyou Guo
AU - Jung U. Shin
AU - Colin A. Jahoda
AU - Angela M. Christiano
AB - Human skin constructs (HSCs) have the potential to provide an effective therapy for patients with significant skin injuries and to enable human-relevant drug screening for skin diseases; however, the incorporation of engineered skin appendages, such as hair follicles (HFs), into HSCs remains a major challenge. Here, we demonstrate a biomimetic approach for generation of human HFs within HSCs by recapitulating the physiological 3D organization of cells in the HF microenvironment using 3D-printed molds. Overexpression of Lef-1 in dermal papilla cells (DPC) restores the intact DPC transcriptional signature and significantly enhances the efficiency of HF differentiation in HSCs. Furthermore, vascularization of hair-bearing HSCs prior to engraftment allows for efficient human hair growth in immunodeficient mice. The ability to regenerate an entire HF from cultured human cells will have a transformative impact on the medical management of different types of alopecia, as well as chronic wounds, which represent major unmet medical needs.
BT - Nature Communications
DA - 2018-12-13
DO - 10.1038/s41467-018-07579-y
IS - 1
LA - en
N2 - Human skin constructs (HSCs) have the potential to provide an effective therapy for patients with significant skin injuries and to enable human-relevant drug screening for skin diseases; however, the incorporation of engineered skin appendages, such as hair follicles (HFs), into HSCs remains a major challenge. Here, we demonstrate a biomimetic approach for generation of human HFs within HSCs by recapitulating the physiological 3D organization of cells in the HF microenvironment using 3D-printed molds. Overexpression of Lef-1 in dermal papilla cells (DPC) restores the intact DPC transcriptional signature and significantly enhances the efficiency of HF differentiation in HSCs. Furthermore, vascularization of hair-bearing HSCs prior to engraftment allows for efficient human hair growth in immunodeficient mice. The ability to regenerate an entire HF from cultured human cells will have a transformative impact on the medical management of different types of alopecia, as well as chronic wounds, which represent major unmet medical needs.
PY - 2018
EP - 5301
T2 - Nature Communications
TI - Tissue engineering of human hair follicles using a biomimetic developmental approach
UR - https://www.nature.com/articles/s41467-018-07579-y
VL - 9
Y2 - 2024-07-30
SN - 2041-1723
ER -