TY - JOUR
KW - Antimicrobial responses
KW - Chemokines
KW - Microbial communities
KW - Tissue engineering
AU - Layla J. Barkal
AU - Clare L. Procknow
AU - Yasmín R. Álvarez-García
AU - Mengyao Niu
AU - José A. Jiménez-Torres
AU - Rebecca A. Brockman-Schneider
AU - James E. Gern
AU - Loren C. Denlinger
AU - Ashleigh B. Theberge
AU - Nancy P. Keller
AU - Erwin Berthier
AU - David J. Beebe
AB - We inhale respiratory pathogens continuously, and the subsequent signaling events between host and microbe are complex, ultimately resulting in clearance of the microbe, stable colonization of the host, or active disease. Traditional in vitro methods are ill-equipped to study these critical events in the context of the lung microenvironment. Here we introduce a microscale organotypic model of the human bronchiole for studying pulmonary infection. By leveraging microscale techniques, the model is designed to approximate the structure of the human bronchiole, containing airway, vascular, and extracellular matrix compartments. To complement direct infection of the organotypic bronchiole, we present a clickable extension that facilitates volatile compound communication between microbial populations and the host model. Using Aspergillus fumigatus, a respiratory pathogen, we characterize the inflammatory response of the organotypic bronchiole to infection. Finally, we demonstrate multikingdom, volatile-mediated communication between the organotypic bronchiole and cultures of Aspergillus fumigatus and Pseudomonas aeruginosa.
BT - Nature Communications
DA - 2017-11-24
DO - 10.1038/s41467-017-01985-4
IS - 1
LA - en
N2 - We inhale respiratory pathogens continuously, and the subsequent signaling events between host and microbe are complex, ultimately resulting in clearance of the microbe, stable colonization of the host, or active disease. Traditional in vitro methods are ill-equipped to study these critical events in the context of the lung microenvironment. Here we introduce a microscale organotypic model of the human bronchiole for studying pulmonary infection. By leveraging microscale techniques, the model is designed to approximate the structure of the human bronchiole, containing airway, vascular, and extracellular matrix compartments. To complement direct infection of the organotypic bronchiole, we present a clickable extension that facilitates volatile compound communication between microbial populations and the host model. Using Aspergillus fumigatus, a respiratory pathogen, we characterize the inflammatory response of the organotypic bronchiole to infection. Finally, we demonstrate multikingdom, volatile-mediated communication between the organotypic bronchiole and cultures of Aspergillus fumigatus and Pseudomonas aeruginosa.
PY - 2017
EP - 1770
T2 - Nature Communications
TI - Microbial volatile communication in human organotypic lung models
UR - https://www.nature.com/articles/s41467-017-01985-4
VL - 8
Y2 - 2024-07-30
SN - 2041-1723
ER -