TY - JOUR
KW - Biotechnology
KW - Cell biology
AU - Pauline Zamprogno
AU - Simon Wüthrich
AU - Sven Achenbach
AU - Giuditta Thoma
AU - Janick D. Stucki
AU - Nina Hobi
AU - Nicole Schneider-Daum
AU - Claus-Michael Lehr
AU - Hanno Huwer
AU - Thomas Geiser
AU - Ralph A. Schmid
AU - Olivier T. Guenat
AB - The air-blood barrier with its complex architecture and dynamic environment is difficult to mimic in vitro. Lung-on-a-chips enable mimicking the breathing movements using a thin, stretchable PDMS membrane. However, they fail to reproduce the characteristic alveoli network as well as the biochemical and physical properties of the alveolar basal membrane. Here, we present a lung-on-a-chip, based on a biological, stretchable and biodegradable membrane made of collagen and elastin, that emulates an array of tiny alveoli with in vivo-like dimensions. This membrane outperforms PDMS in many ways: it does not absorb rhodamine-B, is biodegradable, is created by a simple method, and can easily be tuned to modify its thickness, composition and stiffness. The air-blood barrier is reconstituted using primary lung alveolar epithelial cells from patients and primary lung endothelial cells. Typical alveolar epithelial cell markers are expressed, while the barrier properties are preserved for up to 3 weeks.
BT - Communications Biology
DA - 2021-02-05
DO - 10.1038/s42003-021-01695-0
IS - 1
LA - en
N2 - The air-blood barrier with its complex architecture and dynamic environment is difficult to mimic in vitro. Lung-on-a-chips enable mimicking the breathing movements using a thin, stretchable PDMS membrane. However, they fail to reproduce the characteristic alveoli network as well as the biochemical and physical properties of the alveolar basal membrane. Here, we present a lung-on-a-chip, based on a biological, stretchable and biodegradable membrane made of collagen and elastin, that emulates an array of tiny alveoli with in vivo-like dimensions. This membrane outperforms PDMS in many ways: it does not absorb rhodamine-B, is biodegradable, is created by a simple method, and can easily be tuned to modify its thickness, composition and stiffness. The air-blood barrier is reconstituted using primary lung alveolar epithelial cells from patients and primary lung endothelial cells. Typical alveolar epithelial cell markers are expressed, while the barrier properties are preserved for up to 3 weeks.
PY - 2021
SP - 1
EP - 10
T2 - Communications Biology
TI - Second-generation lung-on-a-chip with an array of stretchable alveoli made with a biological membrane
UR - https://www.nature.com/articles/s42003-021-01695-0
VL - 4
Y2 - 2024-07-30
SN - 2399-3642
ER -