TY - JOUR
KW - ex vivo
KW - infection
KW - organotypic models
KW - precision-cut lung slices
KW - respiratory infections
AU - Flávia Viana
AU - Cecilia M. O’Kane
AU - Gunnar N. Schroeder
AB - Respiratory infections are a leading cause of mortality worldwide. Most of the research on the underlying disease mechanisms is based on cell culture, organoid, or surrogate animal models. Although these provide important insights, they have limitations. Cell culture models fail to recapitulate cellular interactions in the lung and animal models often do not permit high-throughput analysis of drugs or pathogen isolates; hence, there is a need for improved, scalable models. Precision-cut lung slices (PCLS), small, uniform tissue slices generated from animal or human lungs are increasingly recognized and employed as an ex vivo organotypic model. PCLS retain remarkable cellular complexity and the architecture of the lung, providing a platform to investigate respiratory pathogens in a near-native environment. Here, we review the generation and features of PCLS, their use to investigate the pathogenesis of viral and bacterial pathogens, and highlight their potential to advance respiratory infection research in the future.
BT - Molecular Microbiology
DA - 2022
DO - 10.1111/mmi.14817
IS - 3
LA - en
N2 - Respiratory infections are a leading cause of mortality worldwide. Most of the research on the underlying disease mechanisms is based on cell culture, organoid, or surrogate animal models. Although these provide important insights, they have limitations. Cell culture models fail to recapitulate cellular interactions in the lung and animal models often do not permit high-throughput analysis of drugs or pathogen isolates; hence, there is a need for improved, scalable models. Precision-cut lung slices (PCLS), small, uniform tissue slices generated from animal or human lungs are increasingly recognized and employed as an ex vivo organotypic model. PCLS retain remarkable cellular complexity and the architecture of the lung, providing a platform to investigate respiratory pathogens in a near-native environment. Here, we review the generation and features of PCLS, their use to investigate the pathogenesis of viral and bacterial pathogens, and highlight their potential to advance respiratory infection research in the future.
PY - 2022
SP - 578
EP - 588
ST - Precision-cut lung slices
T2 - Molecular Microbiology
TI - Precision-cut lung slices: A powerful ex vivo model to investigate respiratory infectious diseases
UR - https://onlinelibrary.wiley.com/doi/abs/10.1111/mmi.14817
VL - 117
Y2 - 2024-07-30
SN - 1365-2958
ER -