TY - JOUR
KW - Atlases as Topic
KW - Chromatin
KW - Fetus
KW - Gene Expression Profiling
KW - Gene Expression Regulation, Developmental
KW - Humans
KW - Neurons
KW - Single-Cell Analysis
KW - Transcription Factors
AU - Junyue Cao
AU - Diana R. O'Day
AU - Hannah A. Pliner
AU - Paul D. Kingsley
AU - Mei Deng
AU - Riza M. Daza
AU - Michael A. Zager
AU - Kimberly A. Aldinger
AU - Ronnie Blecher-Gonen
AU - Fan Zhang
AU - Malte Spielmann
AU - James Palis
AU - Dan Doherty
AU - Frank J. Steemers
AU - Ian A. Glass
AU - Cole Trapnell
AU - Jay Shendure
AB - The gene expression program underlying the specification of human cell types is of fundamental interest. We generated human cell atlases of gene expression and chromatin accessibility in fetal tissues. For gene expression, we applied three-level combinatorial indexing to >110 samples representing 15 organs, ultimately profiling ~4 million single cells. We leveraged the literature and other atlases to identify and annotate hundreds of cell types and subtypes, both within and across tissues. Our analyses focused on organ-specific specializations of broadly distributed cell types (such as blood, endothelial, and epithelial), sites of fetal erythropoiesis (which notably included the adrenal gland), and integration with mouse developmental atlases (such as conserved specification of blood cells). These data represent a rich resource for the exploration of in vivo human gene expression in diverse tissues and cell types.
BT - Science (New York, N.Y.)
DA - 2020-11-13
DO - 10.1126/science.aba7721
IS - 6518
LA - eng
N2 - The gene expression program underlying the specification of human cell types is of fundamental interest. We generated human cell atlases of gene expression and chromatin accessibility in fetal tissues. For gene expression, we applied three-level combinatorial indexing to >110 samples representing 15 organs, ultimately profiling ~4 million single cells. We leveraged the literature and other atlases to identify and annotate hundreds of cell types and subtypes, both within and across tissues. Our analyses focused on organ-specific specializations of broadly distributed cell types (such as blood, endothelial, and epithelial), sites of fetal erythropoiesis (which notably included the adrenal gland), and integration with mouse developmental atlases (such as conserved specification of blood cells). These data represent a rich resource for the exploration of in vivo human gene expression in diverse tissues and cell types.
PY - 2020
EP - eaba7721
T2 - Science (New York, N.Y.)
TI - A human cell atlas of fetal gene expression
VL - 370
SN - 1095-9203
ER -