TY - JOUR
AU - Louise A. Huuki-Myers
AU - Abby Spangler
AU - Nicholas J. Eagles
AU - Kelsey D. Montgomery
AU - Sang Ho Kwon
AU - Boyi Guo
AU - Melissa Grant-Peters
AU - Heena R. Divecha
AU - Madhavi Tippani
AU - Chaichontat Sriworarat
AU - Annie B. Nguyen
AU - Prashanthi Ravichandran
AU - Matthew N. Tran
AU - Arta Seyedian
AU - PsychENCODE Consortium
AU - Thomas M. Hyde
AU - Joel E. Kleinman
AU - Alexis Battle
AU - Stephanie C. Page
AU - Mina Ryten
AU - Stephanie C. Hicks
AU - Keri Martinowich
AU - Leonardo Collado-Torres
AU - Kristen R. Maynard
AB - The molecular organization of the human neocortex historically has been studied in the context of its histological layers. However, emerging spatial transcriptomic technologies have enabled unbiased identification of transcriptionally defined spatial domains that move beyond classic cytoarchitecture. We used the Visium spatial gene expression platform to generate a data-driven molecular neuroanatomical atlas across the anterior-posterior axis of the human dorsolateral prefrontal cortex. Integration with paired single-nucleus RNA-sequencing data revealed distinct cell type compositions and cell-cell interactions across spatial domains. Using PsychENCODE and publicly available data, we mapped the enrichment of cell types and genes associated with neuropsychiatric disorders to discrete spatial domains.
BT - Science
DA - 2024-05-24
DO - 10.1126/science.adh1938
IS - 6698
N2 - The molecular organization of the human neocortex historically has been studied in the context of its histological layers. However, emerging spatial transcriptomic technologies have enabled unbiased identification of transcriptionally defined spatial domains that move beyond classic cytoarchitecture. We used the Visium spatial gene expression platform to generate a data-driven molecular neuroanatomical atlas across the anterior-posterior axis of the human dorsolateral prefrontal cortex. Integration with paired single-nucleus RNA-sequencing data revealed distinct cell type compositions and cell-cell interactions across spatial domains. Using PsychENCODE and publicly available data, we mapped the enrichment of cell types and genes associated with neuropsychiatric disorders to discrete spatial domains.
PY - 2024
EP - eadh1938
T2 - Science
TI - A data-driven single-cell and spatial transcriptomic map of the human prefrontal cortex
UR - https://www.science.org/doi/10.1126/science.adh1938
VL - 384
Y2 - 2024-06-18
ER -