TY - JOUR
AU - Soraya O. Sandoval
AU - Gerarda Cappuccio
AU - Karina Kruth
AU - Sivan Osenberg
AU - Saleh M. Khalil
AU - Natasha M. Méndez-Albelo
AU - Krishnan Padmanabhan
AU - Daifeng Wang
AU - Mark J. Niciu
AU - Anita Bhattacharyya
AU - Jason L. Stein
AU - André M. M. Sousa
AU - Elisa A. Waxman
AU - Elizabeth D. Buttermore
AU - Dosh Whye
AU - Carissa L. Sirois
AU - Stewart Anderson
AU - Anita
AU - Anita Bhattacharyya
AU - Elizabeth Buttermore
AU - Qiang Chang
AU - Deborah French
AU - Kazue Hashimoto-Torii
AU - Harley Kornblum
AU - Kristin Kroll
AU - Herbert M. Lachman
AU - Mirjana Maletic-Savatic
AU - Mark Niciu
AU - Bennett Novitch
AU - Krishnan Padmanabhan
AU - Chris Proschel
AU - Mustafa Sahin
AU - Andre Sousa
AU - Jason Stein
AU - Daifeng Wang
AU - Elisa Waxman
AU - Dosh Whye
AU - Aislinn Williams
AU - Xinyu Zhao
AU - Aislinn Williams
AU - Mirjana Maletic-Savatic
AU - Xinyu Zhao
AB - Human brain organoid models have emerged as a promising tool for studying human brain development and function. These models preserve human genetics and recapitulate some aspects of human brain development, while facilitating manipulation in an in vitro setting. Despite their potential to transform biology and medicine, concerns persist about their fidelity. To fully harness their potential, it is imperative to establish reliable analytic methods, ensuring rigor and reproducibility. Here, we review current analytical platforms used to characterize human forebrain cortical organoids, highlight challenges, and propose recommendations for future studies to achieve greater precision and uniformity across laboratories.
BT - Stem Cell Reports
DA - 2024-06-11
DO - 10.1016/j.stemcr.2024.04.008
IS - 6
N2 - Human brain organoid models have emerged as a promising tool for studying human brain development and function. These models preserve human genetics and recapitulate some aspects of human brain development, while facilitating manipulation in an in vitro setting. Despite their potential to transform biology and medicine, concerns persist about their fidelity. To fully harness their potential, it is imperative to establish reliable analytic methods, ensuring rigor and reproducibility. Here, we review current analytical platforms used to characterize human forebrain cortical organoids, highlight challenges, and propose recommendations for future studies to achieve greater precision and uniformity across laboratories.
PY - 2024
SP - 796
EP - 816
ST - Rigor and reproducibility in human brain organoid research
T2 - Stem Cell Reports
TI - Rigor and reproducibility in human brain organoid research: Where we are and where we need to go
UR - https://www.sciencedirect.com/science/article/pii/S2213671124001140
VL - 19
Y2 - 2024-06-18
SN - 2213-6711
ER -