TY - JOUR
KW - Cellular neuroscience
KW - Short-term potentiation
KW - Synaptic development
KW - Tissue engineering
AU - Tatsuya Osaki
AU - Tomoya Duenki
AU - Siu Yu A. Chow
AU - Yasuhiro Ikegami
AU - Romain Beaubois
AU - Timothée Levi
AU - Nao Nakagawa-Tamagawa
AU - Yoji Hirano
AU - Yoshiho Ikeuchi
AB - An inter-regional cortical tract is one of the most fundamental architectural motifs that integrates neural circuits to orchestrate and generate complex functions of the human brain. To understand the mechanistic significance of inter-regional projections on development of neural circuits, we investigated an in vitro neural tissue model for inter-regional connections, in which two cerebral organoids are connected with a bundle of reciprocally extended axons. The connected organoids produced more complex and intense oscillatory activity than conventional or directly fused cerebral organoids, suggesting the inter-organoid axonal connections enhance and support the complex network activity. In addition, optogenetic stimulation of the inter-organoid axon bundles could entrain the activity of the organoids and induce robust short-term plasticity of the macroscopic circuit. These results demonstrated that the projection axons could serve as a structural hub that boosts functionality of the organoid-circuits. This model could contribute to further investigation on development and functions of macroscopic neuronal circuits in vitro.
BT - Nature Communications
DA - 2024-04-10
DO - 10.1038/s41467-024-46787-7
IS - 1
LA - en
N2 - An inter-regional cortical tract is one of the most fundamental architectural motifs that integrates neural circuits to orchestrate and generate complex functions of the human brain. To understand the mechanistic significance of inter-regional projections on development of neural circuits, we investigated an in vitro neural tissue model for inter-regional connections, in which two cerebral organoids are connected with a bundle of reciprocally extended axons. The connected organoids produced more complex and intense oscillatory activity than conventional or directly fused cerebral organoids, suggesting the inter-organoid axonal connections enhance and support the complex network activity. In addition, optogenetic stimulation of the inter-organoid axon bundles could entrain the activity of the organoids and induce robust short-term plasticity of the macroscopic circuit. These results demonstrated that the projection axons could serve as a structural hub that boosts functionality of the organoid-circuits. This model could contribute to further investigation on development and functions of macroscopic neuronal circuits in vitro.
PY - 2024
EP - 2945
T2 - Nature Communications
TI - Complex activity and short-term plasticity of human cerebral organoids reciprocally connected with axons
UR - https://www.nature.com/articles/s41467-024-46787-7
VL - 15
Y2 - 2024-04-10
SN - 2041-1723
ER -