TY - JOUR
KW - Diabetes Mellitus, Type 2
KW - Drug Discovery
KW - Exenatide
KW - Glucose
KW - Humans
KW - Hypoglycemic Agents
KW - Insulin
KW - Islets of Langerhans
KW - Microfluidic Analytical Techniques
KW - Models, Biological
KW - Tissue Array Analysis
KW - Tolbutamide
KW - Diabetes
KW - glucose-stimulated insulin secretion
KW - hanging-drops
KW - organ on chip
KW - pancreatic islets
AU - Patrick M. Misun
AU - Burçak Yesildag
AU - Felix Forschler
AU - Aparna Neelakandhan
AU - Nassim Rousset
AU - Adelinn Biernath
AU - Andreas Hierlemann
AU - Olivier Frey
AB - Insulin is released from pancreatic islets in a biphasic and pulsatile manner in response to elevated glucose levels. This highly dynamic insulin release can be studied in vitro with islet perifusion assays. Herein, a novel platform to perform glucose-stimulated insulin secretion (GSIS) assays with single islets is presented for studying the dynamics of insulin release at high temporal resolution. A standardized human islet model is developed and a microfluidic hanging-drop-based perifusion system is engineered, which facilitates rapid glucose switching, minimal sample dilution, low analyte dispersion, and short sampling intervals. Human islet microtissues feature robust and long-term glucose responsiveness and demonstrate reproducible dynamic GSIS with a prominent first phase and a sustained, pulsatile second phase. Perifusion of single islet microtissues produces a higher peak secretion rate, higher secretion during the first and second phases of insulin release, as well as more defined pulsations during the second phase in comparison to perifusion of pooled islets. The developed platform enables to study compound effects on both phases of insulin secretion as shown with two classes of insulin secretagogs. It provides a new tool for studying physiologically relevant dynamic insulin secretion at comparably low sample-to-sample variation and high temporal resolution.
BT - Advanced Biosystems
DA - 2020-03
DO - 10.1002/adbi.201900291
IS - 3
LA - eng
N2 - Insulin is released from pancreatic islets in a biphasic and pulsatile manner in response to elevated glucose levels. This highly dynamic insulin release can be studied in vitro with islet perifusion assays. Herein, a novel platform to perform glucose-stimulated insulin secretion (GSIS) assays with single islets is presented for studying the dynamics of insulin release at high temporal resolution. A standardized human islet model is developed and a microfluidic hanging-drop-based perifusion system is engineered, which facilitates rapid glucose switching, minimal sample dilution, low analyte dispersion, and short sampling intervals. Human islet microtissues feature robust and long-term glucose responsiveness and demonstrate reproducible dynamic GSIS with a prominent first phase and a sustained, pulsatile second phase. Perifusion of single islet microtissues produces a higher peak secretion rate, higher secretion during the first and second phases of insulin release, as well as more defined pulsations during the second phase in comparison to perifusion of pooled islets. The developed platform enables to study compound effects on both phases of insulin secretion as shown with two classes of insulin secretagogs. It provides a new tool for studying physiologically relevant dynamic insulin secretion at comparably low sample-to-sample variation and high temporal resolution.
PY - 2020
EP - e1900291
T2 - Advanced Biosystems
TI - In Vitro Platform for Studying Human Insulin Release Dynamics of Single Pancreatic Islet Microtissues at High Resolution
VL - 4
SN - 2366-7478
ER -