TY - JOUR
KW - Biomaterials – cells
KW - Cardiovascular models
AU - Suji Choi
AU - Keel Yong Lee
AU - Sean L. Kim
AU - Luke A. MacQueen
AU - Huibin Chang
AU - John F. Zimmerman
AU - Qianru Jin
AU - Michael M. Peters
AU - Herdeline Ann M. Ardoña
AU - Xujie Liu
AU - Ann-Caroline Heiler
AU - Rudy Gabardi
AU - Collin Richardson
AU - William T. Pu
AU - Andreas R. Bausch
AU - Kevin Kit Parker
AB - Hydrogels are attractive materials for tissue engineering, but efforts to date have shown limited ability to produce the microstructural features necessary to promote cellular self-organization into hierarchical three-dimensional (3D) organ models. Here we develop a hydrogel ink containing prefabricated gelatin fibres to print 3D organ-level scaffolds that recapitulate the intra- and intercellular organization of the heart. The addition of prefabricated gelatin fibres to hydrogels enables the tailoring of the ink rheology, allowing for a controlled sol–gel transition to achieve precise printing of free-standing 3D structures without additional supporting materials. Shear-induced alignment of fibres during ink extrusion provides microscale geometric cues that promote the self-organization of cultured human cardiomyocytes into anisotropic muscular tissues in vitro. The resulting 3D-printed ventricle in vitro model exhibited biomimetic anisotropic electrophysiological and contractile properties.
BT - Nature Materials
DA - 2023-08
DO - 10.1038/s41563-023-01611-3
IS - 8
LA - en
N2 - Hydrogels are attractive materials for tissue engineering, but efforts to date have shown limited ability to produce the microstructural features necessary to promote cellular self-organization into hierarchical three-dimensional (3D) organ models. Here we develop a hydrogel ink containing prefabricated gelatin fibres to print 3D organ-level scaffolds that recapitulate the intra- and intercellular organization of the heart. The addition of prefabricated gelatin fibres to hydrogels enables the tailoring of the ink rheology, allowing for a controlled sol–gel transition to achieve precise printing of free-standing 3D structures without additional supporting materials. Shear-induced alignment of fibres during ink extrusion provides microscale geometric cues that promote the self-organization of cultured human cardiomyocytes into anisotropic muscular tissues in vitro. The resulting 3D-printed ventricle in vitro model exhibited biomimetic anisotropic electrophysiological and contractile properties.
PY - 2023
SP - 1039
EP - 1046
T2 - Nature Materials
TI - Fibre-infused gel scaffolds guide cardiomyocyte alignment in 3D-printed ventricles
UR - https://www.nature.com/articles/s41563-023-01611-3
VL - 22
Y2 - 2023-09-15
SN - 1476-4660
ER -