TY - JOUR
KW - Animals
KW - Betacoronavirus
KW - COVID-19
KW - Cell Differentiation
KW - Cells, Cultured
KW - Child, Preschool
KW - Chiroptera
KW - Chlorocebus aethiops
KW - Coronavirus Infections
KW - enterocytes
KW - Female
KW - Humans
KW - Infant
KW - Intestinal Mucosa
KW - Intestines
KW - Male
KW - organoids
KW - Pandemics
KW - Pneumonia, Viral
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - SARS-CoV-2
KW - Vero Cells
KW - Viral Load
KW - Viral Tropism
AU - Jie Zhou
AU - Cun Li
AU - Xiaojuan Liu
AU - Man Chun Chiu
AU - Xiaoyu Zhao
AU - Dong Wang
AU - Yuxuan Wei
AU - Andrew Lee
AU - Anna Jinxia Zhang
AU - Hin Chu
AU - Jian-Piao Cai
AU - Cyril Chik-Yan Yip
AU - Ivy Hau-Yee Chan
AU - Kenneth Kak-Yuen Wong
AU - Owen Tak-Yin Tsang
AU - Kwok-Hung Chan
AU - Jasper Fuk-Woo Chan
AU - Kelvin Kai-Wang To
AU - Honglin Chen
AU - Kwok Yung Yuen
AB - A novel coronavirus-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-emerged in humans in Wuhan, China, in December 2019 and has since disseminated globally1,2. As of April 16, 2020, the confirmed case count of coronavirus disease 2019 (COVID-19) had surpassed 2 million. Based on full-genome sequence analysis, SARS-CoV-2 shows high homology to SARS-related coronaviruses identified in horseshoe bats1,2. Here we show the establishment and characterization of expandable intestinal organoids derived from horseshoe bats of the Rhinolophus sinicus species that can recapitulate bat intestinal epithelium. These bat enteroids are fully susceptible to SARS-CoV-2 infection and sustain robust viral replication. Development of gastrointestinal symptoms in some patients with COVID-19 and detection of viral RNA in fecal specimens suggest that SARS-CoV-2 might cause enteric, in addition to respiratory, infection3,4. Here we demonstrate active replication of SARS-CoV-2 in human intestinal organoids and isolation of infectious virus from the stool specimen of a patient with diarrheal COVID-19. Collectively, we established the first expandable organoid culture system of bat intestinal epithelium and present evidence that SARS-CoV-2 can infect bat intestinal cells. The robust SARS-CoV-2 replication in human intestinal organoids suggests that the human intestinal tract might be a transmission route of SARS-CoV-2.
BT - Nature Medicine
DA - 2020-07
DO - 10.1038/s41591-020-0912-6
IS - 7
LA - eng
N2 - A novel coronavirus-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-emerged in humans in Wuhan, China, in December 2019 and has since disseminated globally1,2. As of April 16, 2020, the confirmed case count of coronavirus disease 2019 (COVID-19) had surpassed 2 million. Based on full-genome sequence analysis, SARS-CoV-2 shows high homology to SARS-related coronaviruses identified in horseshoe bats1,2. Here we show the establishment and characterization of expandable intestinal organoids derived from horseshoe bats of the Rhinolophus sinicus species that can recapitulate bat intestinal epithelium. These bat enteroids are fully susceptible to SARS-CoV-2 infection and sustain robust viral replication. Development of gastrointestinal symptoms in some patients with COVID-19 and detection of viral RNA in fecal specimens suggest that SARS-CoV-2 might cause enteric, in addition to respiratory, infection3,4. Here we demonstrate active replication of SARS-CoV-2 in human intestinal organoids and isolation of infectious virus from the stool specimen of a patient with diarrheal COVID-19. Collectively, we established the first expandable organoid culture system of bat intestinal epithelium and present evidence that SARS-CoV-2 can infect bat intestinal cells. The robust SARS-CoV-2 replication in human intestinal organoids suggests that the human intestinal tract might be a transmission route of SARS-CoV-2.
PY - 2020
SP - 1077
EP - 1083
T2 - Nature Medicine
TI - Infection of bat and human intestinal organoids by SARS-CoV-2
VL - 26
SN - 1546-170X
ER -