TY - JOUR
KW - Angiotensin-Converting Enzyme 2
KW - Betacoronavirus
KW - Cell Culture Techniques
KW - Cell Differentiation
KW - Cell Lineage
KW - Cell Proliferation
KW - Culture Media
KW - enterocytes
KW - Gene expression
KW - Humans
KW - Ileum
KW - Lung
KW - Male
KW - organoids
KW - Peptidyl-Dipeptidase A
KW - RNA, Messenger
KW - Receptors, Virus
KW - Respiratory Mucosa
KW - SARS-CoV-2
KW - Severe acute respiratory syndrome-related coronavirus
KW - Virus Replication
AU - Mart M. Lamers
AU - Joep Beumer
AU - Jelte van der Vaart
AU - Kèvin Knoops
AU - Jens Puschhof
AU - Tim I. Breugem
AU - Raimond B. G. Ravelli
AU - J. Paul van Schayck
AU - Anna Z. Mykytyn
AU - Hans Q. Duimel
AU - Elly van Donselaar
AU - Samra Riesebosch
AU - Helma J. H. Kuijpers
AU - Debby Schipper
AU - Willine J. van de Wetering
AU - Miranda de Graaf
AU - Marion Koopmans
AU - Edwin Cuppen
AU - Peter J. Peters
AU - Bart L. Haagmans
AU - Hans Clevers
AB - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can cause coronavirus disease 2019 (COVID-19), an influenza-like disease that is primarily thought to infect the lungs with transmission through the respiratory route. However, clinical evidence suggests that the intestine may present another viral target organ. Indeed, the SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) is highly expressed on differentiated enterocytes. In human small intestinal organoids (hSIOs), enterocytes were readily infected by SARS-CoV and SARS-CoV-2, as demonstrated by confocal and electron microscopy. Enterocytes produced infectious viral particles, whereas messenger RNA expression analysis of hSIOs revealed induction of a generic viral response program. Therefore, the intestinal epithelium supports SARS-CoV-2 replication, and hSIOs serve as an experimental model for coronavirus infection and biology.
BT - Science (New York, N.Y.)
DA - 2020-07-03
DO - 10.1126/science.abc1669
IS - 6499
LA - eng
N2 - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can cause coronavirus disease 2019 (COVID-19), an influenza-like disease that is primarily thought to infect the lungs with transmission through the respiratory route. However, clinical evidence suggests that the intestine may present another viral target organ. Indeed, the SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) is highly expressed on differentiated enterocytes. In human small intestinal organoids (hSIOs), enterocytes were readily infected by SARS-CoV and SARS-CoV-2, as demonstrated by confocal and electron microscopy. Enterocytes produced infectious viral particles, whereas messenger RNA expression analysis of hSIOs revealed induction of a generic viral response program. Therefore, the intestinal epithelium supports SARS-CoV-2 replication, and hSIOs serve as an experimental model for coronavirus infection and biology.
PY - 2020
SP - 50
EP - 54
T2 - Science (New York, N.Y.)
TI - SARS-CoV-2 productively infects human gut enterocytes
VL - 369
SN - 1095-9203
ER -