TY - JOUR
AU - Mart M. Lamers
AU - Tim I. Breugem
AU - Anna Z. Mykytyn
AU - Yiquan Wang
AU - Nathalie Groen
AU - Kèvin Knoops
AU - Debby Schipper
AU - Jelte van der Vaart
AU - Charlotte D. Koopman
AU - Jingshu Zhang
AU - Douglas C. Wu
AU - Petra B. van den Doel
AU - Theo Bestebroer
AU - Corine H. GeurtsvanKessel
AU - Peter J. Peters
AU - Mauro J. Muraro
AU - Hans Clevers
AU - Nicholas C. Wu
AU - Bart L. Haagmans
AB - A new phase of the COVID-19 pandemic has started as several SARS-CoV-2 variants are rapidly emerging globally, raising concerns for increased transmissibility. As animal models and traditional in vitro systems may fail to model key aspects of the SARS-CoV-2 replication cycle, representative in vitro systems to assess variants phenotypically are urgently needed. We found that the British variant (clade B.1.1.7), compared to an ancestral SARS-CoV-2 clade B virus, produced higher levels of infectious virus late in infection and had a higher replicative fitness in human airway, alveolar and intestinal organoid models. Our findings unveil human organoids as powerful tools to phenotype viral variants and suggest extended shedding as a correlate of fitness for SARS-CoV-2.
One-Sentence Summary British SARS-CoV-2 variant (clade B.1.1.7) infects organoids for extended time and has a higher fitness in vitro.
DA - 2021-05-03
DO - 10.1101/2021.05.03.441080
LA - en
N2 - A new phase of the COVID-19 pandemic has started as several SARS-CoV-2 variants are rapidly emerging globally, raising concerns for increased transmissibility. As animal models and traditional in vitro systems may fail to model key aspects of the SARS-CoV-2 replication cycle, representative in vitro systems to assess variants phenotypically are urgently needed. We found that the British variant (clade B.1.1.7), compared to an ancestral SARS-CoV-2 clade B virus, produced higher levels of infectious virus late in infection and had a higher replicative fitness in human airway, alveolar and intestinal organoid models. Our findings unveil human organoids as powerful tools to phenotype viral variants and suggest extended shedding as a correlate of fitness for SARS-CoV-2.
One-Sentence Summary British SARS-CoV-2 variant (clade B.1.1.7) infects organoids for extended time and has a higher fitness in vitro.
PY - 2021
TI - Human organoid systems reveal in vitro correlates of fitness for SARS-CoV-2 B.1.1.7
UR - https://www.biorxiv.org/content/10.1101/2021.05.03.441080v1
Y2 - 2023-08-09
ER -