TY - JOUR
AU - Ruochen Zang
AU - Maria Florencia Gomez Castro
AU - Broc T. McCune
AU - Qiru Zeng
AU - Paul W. Rothlauf
AU - Naomi M. Sonnek
AU - Zhuoming Liu
AU - Kevin F. Brulois
AU - Xin Wang
AU - Harry B. Greenberg
AU - Michael S. Diamond
AU - Matthew A. Ciorba
AU - Sean P. J. Whelan
AU - Siyuan Ding
AB - Gastrointestinal symptoms and fecal shedding of SARS-CoV-2 RNA are frequently observed in COVID-19. However, it is unclear whether SARS-CoV-2 replicates in the human intestine and contributes to possible fecal-oral transmission. Here, we report productive infection of SARS-CoV-2 in ACE2+ mature enterocytes in human small intestinal enteroids. Expression of two mucosa-specific serine proteases, TMPRSS2 and TMPRSS4, facilitated SARS-CoV-2 spike fusogenic activity and promoted virus entry into host cells. We also demonstrate that viruses released into the intestinal lumen were inactivated by simulated human colonic fluid, and infectious virus was not recovered from the stool specimens of patients with COVID-19. Our results highlight the intestine as a potential site of SARS-CoV-2 replication, which may contribute to local and systemic illness and overall disease progression.
BT - Science Immunology
DA - 2020-05-13
DO - 10.1126/sciimmunol.abc3582
IS - 47
N2 - Gastrointestinal symptoms and fecal shedding of SARS-CoV-2 RNA are frequently observed in COVID-19. However, it is unclear whether SARS-CoV-2 replicates in the human intestine and contributes to possible fecal-oral transmission. Here, we report productive infection of SARS-CoV-2 in ACE2+ mature enterocytes in human small intestinal enteroids. Expression of two mucosa-specific serine proteases, TMPRSS2 and TMPRSS4, facilitated SARS-CoV-2 spike fusogenic activity and promoted virus entry into host cells. We also demonstrate that viruses released into the intestinal lumen were inactivated by simulated human colonic fluid, and infectious virus was not recovered from the stool specimens of patients with COVID-19. Our results highlight the intestine as a potential site of SARS-CoV-2 replication, which may contribute to local and systemic illness and overall disease progression.
PY - 2020
EP - eabc3582
T2 - Science Immunology
TI - TMPRSS2 and TMPRSS4 promote SARS-CoV-2 infection of human small intestinal enterocytes
UR - https://www.science.org/doi/10.1126/sciimmunol.abc3582
VL - 5
Y2 - 2023-08-09
ER -