TY - JOUR
KW - Respiratory Tract Diseases
KW - SARS-CoV-2
AU - Emi Sano
AU - Tatsuya Suzuki
AU - Rina Hashimoto
AU - Yumi Itoh
AU - Ayaka Sakamoto
AU - Yusuke Sakai
AU - Akatsuki Saito
AU - Daisuke Okuzaki
AU - Daisuke Motooka
AU - Yukiko Muramoto
AU - Takeshi Noda
AU - Tomohiko Takasaki
AU - Jun-Ichi Sakuragi
AU - Shohei Minami
AU - Takeshi Kobayashi
AU - Takuya Yamamoto
AU - Yasufumi Matsumura
AU - Miki Nagao
AU - Toru Okamoto
AU - Kazuo Takayama
AB - The development of an in vitro cell model that can be used to study severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) research is expected. Here we conducted infection experiments in bronchial organoids (BO) and an BO-derived air-liquid interface model (BO-ALI) using 8 SARS-CoV-2 variants. The infection efficiency in BO-ALI was more than 1,000 times higher than that in BO. Among the bronchial epithelial cells, we found that ciliated cells were infected with the virus, but basal cells were not. Ciliated cells died 7 days after the viral infection, but basal cells survived after the viral infection and differentiated into ciliated cells. Fibroblast growth factor 10 signaling was essential for this differentiation. These results indicate that BO and BO-ALI may be used not only to evaluate the cell response to SARS-CoV-2 and coronavirus disease 2019 (COVID-19) therapeutic agents, but also for airway regeneration studies.
BT - Communications Biology
DA - 2022-05-30
DO - 10.1038/s42003-022-03499-2
IS - 1
LA - en
N2 - The development of an in vitro cell model that can be used to study severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) research is expected. Here we conducted infection experiments in bronchial organoids (BO) and an BO-derived air-liquid interface model (BO-ALI) using 8 SARS-CoV-2 variants. The infection efficiency in BO-ALI was more than 1,000 times higher than that in BO. Among the bronchial epithelial cells, we found that ciliated cells were infected with the virus, but basal cells were not. Ciliated cells died 7 days after the viral infection, but basal cells survived after the viral infection and differentiated into ciliated cells. Fibroblast growth factor 10 signaling was essential for this differentiation. These results indicate that BO and BO-ALI may be used not only to evaluate the cell response to SARS-CoV-2 and coronavirus disease 2019 (COVID-19) therapeutic agents, but also for airway regeneration studies.
PY - 2022
SP - 1
EP - 9
T2 - Communications Biology
TI - Cell response analysis in SARS-CoV-2 infected bronchial organoids
UR - https://www.nature.com/articles/s42003-022-03499-2
VL - 5
Y2 - 2023-08-09
SN - 2399-3642
ER -