TY - JOUR
AU - David Pamies
AU - Marcel Leist
AU - Sandra Coecke
AU - Gerard Bowe
AU - David G. Allen
AU - Gerhard Gstraunthaler
AU - Anna Bal-Price
AU - Francesca Pistollato
AU - Rob B. M. De Vries
AU - Helena T. Hogberg
AU - Thomas Hartung
AU - Glyn Stacey
AB - Good Cell and Tissue Culture Practice (GCCP) 2.0 is an updated guidance document from GCCP 1.0 (published by ECVAM in 2005), which was developed for practical use in the laboratory to assure the reproducibility of in vitro (cell-based) work. The update in the guidance was essential as cell models have advanced dramatically to more complex culture systems and need more comprehensive quality management to ensure reproducibility and high-quality scientific data. This document describes six main principles to consider when performing cell culture including characterization and maintenance of essential characteristics, quality management, documentation and reporting, safety, education and training, and ethics. The document does not intend to impose detailed procedures but to describe potential quality issues. It is foreseen that the document will require further updates as the science and technologies evolve over time.
BT - Alternatives to Animal Experimentation
DA - 2022-01-12
DO - 10.14573/altex.2111011
IS - 1
LA - en
N2 - Good Cell and Tissue Culture Practice (GCCP) 2.0 is an updated guidance document from GCCP 1.0 (published by ECVAM in 2005), which was developed for practical use in the laboratory to assure the reproducibility of in vitro (cell-based) work. The update in the guidance was essential as cell models have advanced dramatically to more complex culture systems and need more comprehensive quality management to ensure reproducibility and high-quality scientific data. This document describes six main principles to consider when performing cell culture including characterization and maintenance of essential characteristics, quality management, documentation and reporting, safety, education and training, and ethics. The document does not intend to impose detailed procedures but to describe potential quality issues. It is foreseen that the document will require further updates as the science and technologies evolve over time.
PY - 2022
SP - 30
EP - 70
T2 - Alternatives to Animal Experimentation
TI - Guidance document on Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0)
UR - https://www.altex.org/index.php/altex/article/view/2376
VL - 39
Y2 - 2023-08-02
SN - 1868-8551
ER -