TY - JOUR
KW - Animal Testing Alternatives
KW - Animals
KW - Coculture Techniques
KW - Gastrointestinal Microbiome
KW - Host Microbial Interactions
KW - Humans
KW - Intestinal Mucosa
KW - Mice
KW - Microbial Interactions
KW - Microchip Analytical Procedures
KW - microfluidics
KW - Models, Animal
KW - organoids
KW - Precision Medicine
KW - bacteria
KW - Co-culture
KW - Epithelium
KW - host
KW - infection
KW - intestine
KW - in vitro models
KW - microbe
KW - microbiota
KW - organ-on-a-chip
KW - organoid
KW - virus
AU - Jens Puschhof
AU - Cayetano Pleguezuelos-Manzano
AU - Hans Clevers
AB - The important and diverse roles of the gut microbiota in human health and disease are increasingly recognized. The difficulty of inferring causation from metagenomic microbiome sequencing studies and from mouse-human interspecies differences has prompted the development of sophisticated in vitro models of human gut-microbe interactions. Here, we review recent advances in the co-culture of microbes with intestinal and colonic epithelia, comparing the rapidly developing fields of organoids and organs-on-chips with other standard models. We describe how specific individual processes by which microbes and epithelia interact can be recapitulated in vitro. Using examples of bacterial, viral, and parasitic infections, we highlight the advantages of each culture model and discuss current trends and future possibilities to build more complex co-cultures.
BT - Cell Host & Microbe
DA - 2021-06-09
DO - 10.1016/j.chom.2021.04.002
IS - 6
LA - eng
N2 - The important and diverse roles of the gut microbiota in human health and disease are increasingly recognized. The difficulty of inferring causation from metagenomic microbiome sequencing studies and from mouse-human interspecies differences has prompted the development of sophisticated in vitro models of human gut-microbe interactions. Here, we review recent advances in the co-culture of microbes with intestinal and colonic epithelia, comparing the rapidly developing fields of organoids and organs-on-chips with other standard models. We describe how specific individual processes by which microbes and epithelia interact can be recapitulated in vitro. Using examples of bacterial, viral, and parasitic infections, we highlight the advantages of each culture model and discuss current trends and future possibilities to build more complex co-cultures.
PY - 2021
SP - 867
EP - 878
ST - Organoids and organs-on-chips
T2 - Cell Host & Microbe
TI - Organoids and organs-on-chips: Insights into human gut-microbe interactions
VL - 29
SN - 1934-6069
ER -