TY - JOUR
KW - Biological Specimen Banks
KW - Carcinogenesis
KW - Coculture Techniques
KW - Gene Editing
KW - Humans
KW - Models, Biological
KW - Neoplasms
KW - organoids
KW - Precision Medicine
KW - Stem cells
KW - Tumor Microenvironment
AU - David Tuveson
AU - Hans Clevers
AB - Organoids are microscopic self-organizing, three-dimensional structures that are grown from stem cells in vitro. They recapitulate many structural and functional aspects of their in vivo counterpart organs. This versatile technology has led to the development of many novel human cancer models. It is now possible to create indefinitely expanding organoids starting from tumor tissue of individuals suffering from a range of carcinomas. Alternatively, CRISPR-based gene modification allows the engineering of organoid models of cancer through the introduction of any combination of cancer gene alterations to normal organoids. When combined with immune cells and fibroblasts, tumor organoids become models for the cancer microenvironment enabling immune-oncology applications. Emerging evidence indicates that organoids can be used to accurately predict drug responses in a personalized treatment setting. Here, we review the current state and future prospects of the rapidly evolving tumor organoid field.
BT - Science (New York, N.Y.)
DA - 2019-06-07
DO - 10.1126/science.aaw6985
IS - 6444
LA - eng
N2 - Organoids are microscopic self-organizing, three-dimensional structures that are grown from stem cells in vitro. They recapitulate many structural and functional aspects of their in vivo counterpart organs. This versatile technology has led to the development of many novel human cancer models. It is now possible to create indefinitely expanding organoids starting from tumor tissue of individuals suffering from a range of carcinomas. Alternatively, CRISPR-based gene modification allows the engineering of organoid models of cancer through the introduction of any combination of cancer gene alterations to normal organoids. When combined with immune cells and fibroblasts, tumor organoids become models for the cancer microenvironment enabling immune-oncology applications. Emerging evidence indicates that organoids can be used to accurately predict drug responses in a personalized treatment setting. Here, we review the current state and future prospects of the rapidly evolving tumor organoid field.
PY - 2019
SP - 952
EP - 955
T2 - Science (New York, N.Y.)
TI - Cancer modeling meets human organoid technology
VL - 364
SN - 1095-9203
ER -