TY - JOUR
KW - Adult
KW - BRCA1 Protein
KW - Breast Neoplasms
KW - Cell Culture Techniques
KW - Cell Differentiation
KW - Cell Lineage
KW - Epidermal Growth Factor
KW - ErbB Receptors
KW - Female
KW - Humans
KW - Mammary Glands, Human
KW - Middle Aged
KW - organoids
KW - Single-Cell Analysis
KW - Stem cells
KW - Transforming Growth Factor beta
KW - Young Adult
KW - p38 Mitogen-Activated Protein Kinases
AU - Jennifer M. Rosenbluth
AU - Ron C. J. Schackmann
AU - G. Kenneth Gray
AU - Laura M. Selfors
AU - Carman Man-Chung Li
AU - Mackenzie Boedicker
AU - Hendrik J. Kuiken
AU - Andrea Richardson
AU - Jane Brock
AU - Judy Garber
AU - Deborah Dillon
AU - Norman Sachs
AU - Hans Clevers
AU - Joan S. Brugge
AB - Recently, organoid technology has been used to generate a large repository of breast cancer organoids. Here we present an extensive evaluation of the ability of organoid culture technology to preserve complex stem/progenitor and differentiated cell types via long-term propagation of normal human mammary tissues. Basal/stem and luminal progenitor cells can differentiate in culture to generate mature basal and luminal cell types, including ER+ cells that have been challenging to maintain in culture. Cells associated with increased cancer risk can also be propagated. Single-cell analyses of matched organoid cultures and native tissues by mass cytometry for 38 markers provide a higher resolution representation of the multiple mammary epithelial cell types in the organoids, and demonstrate that protein expression patterns of the tissue of origin can be preserved in culture. These studies indicate that organoid cultures provide a valuable platform for studies of mammary differentiation, transformation, and breast cancer risk.
BT - Nature Communications
DA - 2020-04-06
DO - 10.1038/s41467-020-15548-7
IS - 1
LA - eng
N2 - Recently, organoid technology has been used to generate a large repository of breast cancer organoids. Here we present an extensive evaluation of the ability of organoid culture technology to preserve complex stem/progenitor and differentiated cell types via long-term propagation of normal human mammary tissues. Basal/stem and luminal progenitor cells can differentiate in culture to generate mature basal and luminal cell types, including ER+ cells that have been challenging to maintain in culture. Cells associated with increased cancer risk can also be propagated. Single-cell analyses of matched organoid cultures and native tissues by mass cytometry for 38 markers provide a higher resolution representation of the multiple mammary epithelial cell types in the organoids, and demonstrate that protein expression patterns of the tissue of origin can be preserved in culture. These studies indicate that organoid cultures provide a valuable platform for studies of mammary differentiation, transformation, and breast cancer risk.
PY - 2020
EP - 1711
T2 - Nature Communications
TI - Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages
VL - 11
SN - 2041-1723
ER -