TY - JOUR
KW - Biosensors
KW - microphysiological systems
KW - organ-on-a-chip
KW - tissue chips
KW - models
AU - Jonathan Sabaté del Río
AU - Jooyoung Ro
AU - Heejeong Yoon
AU - Tae-Eun Park
AU - Yoon-Kyoung Cho
AB - Organs-on-chips (OoCs) are biomimetic in vitro systems based on microfluidic cell cultures that recapitulate the in vivo physicochemical microenvironments and the physiologies and key functional units of specific human organs. These systems are versatile and can be customized to investigate organ-specific physiology, pathology, or pharmacology. They are more physiologically relevant than traditional two-dimensional cultures, can potentially replace the animal models or reduce the use of these models, and represent a unique opportunity for the development of personalized medicine when combined with human induced pluripotent stem cells. Continuous monitoring of important quality parameters of OoCs via a label-free, non-destructive, reliable, high-throughput, and multiplex method is critical for assessing the conditions of these systems and generating relevant analytical data; moreover, elaboration of quality predictive models is required for clinical trials of OoCs. Presently, these analytical data are obtained by manual or automatic sampling and analyzed using single-point, off-chip traditional methods. In this review, we describe recent efforts to integrate biosensing technologies into OoCs for monitoring the physiologies, functions, and physicochemical microenvironments of OoCs. Furthermore, we present potential alternative solutions to current challenges and future directions for the application of artificial intelligence in the development of OoCs and cyber-physical systems. These “smart” OoCs can learn and make autonomous decisions for process optimization, self-regulation, and data analysis.
BT - Biosensors and Bioelectronics
DA - 2023-03-15
DO - 10.1016/j.bios.2022.115057
LA - en
N2 - Organs-on-chips (OoCs) are biomimetic in vitro systems based on microfluidic cell cultures that recapitulate the in vivo physicochemical microenvironments and the physiologies and key functional units of specific human organs. These systems are versatile and can be customized to investigate organ-specific physiology, pathology, or pharmacology. They are more physiologically relevant than traditional two-dimensional cultures, can potentially replace the animal models or reduce the use of these models, and represent a unique opportunity for the development of personalized medicine when combined with human induced pluripotent stem cells. Continuous monitoring of important quality parameters of OoCs via a label-free, non-destructive, reliable, high-throughput, and multiplex method is critical for assessing the conditions of these systems and generating relevant analytical data; moreover, elaboration of quality predictive models is required for clinical trials of OoCs. Presently, these analytical data are obtained by manual or automatic sampling and analyzed using single-point, off-chip traditional methods. In this review, we describe recent efforts to integrate biosensing technologies into OoCs for monitoring the physiologies, functions, and physicochemical microenvironments of OoCs. Furthermore, we present potential alternative solutions to current challenges and future directions for the application of artificial intelligence in the development of OoCs and cyber-physical systems. These “smart” OoCs can learn and make autonomous decisions for process optimization, self-regulation, and data analysis.
PY - 2023
EP - 115057
ST - Integrated technologies for continuous monitoring of organs-on-chips
T2 - Biosensors and Bioelectronics
TI - Integrated technologies for continuous monitoring of organs-on-chips: Current challenges and potential solutions
UR - https://www.sciencedirect.com/science/article/pii/S0956566322010971
VL - 224
Y2 - 2023-07-12
SN - 0956-5663
ER -