01813nas a2200229   4500000000100000000000100001008004100002260001500043653001700058653002700075653001600102653001800118100002500136700002000161700001800181245010300199856005500302300000900357490000700366520119600373022001401569       2025                            d  c2025-02-1910abiomaterials10aBiomedical Engineering10aEngineering10aPharmaceutics1 aRodi Kado Abdalkader1 aSatoshi Konishi1 aTakuya Fujita00aDevelopment of a flexible 3D printed TPU-PVC microfluidic devices for organ-on-a-chip applications  uhttps://www.nature.com/articles/s41598-025-90470-w  a61250 v153 aThe development of cost-effective, flexible, and scalable microfluidic devices is crucial for advancing organ-on-a-chip (OoC) technology for drug discovery and disease modeling applications. In this study, we present a novel 3D-printed flexible microfluidic device (3D-FlexTPU-MFD) fabricated through a one-step fused deposition modeling (FDM) process using thermoplastic polyurethane (TPU) as the printing filament and polyvinyl chloride (PVC) as the bonding substrate. The device’s compatibility was evaluated with various cell types, including human primary myoblasts, human primary endothelial cells (HUVEC), and human iPSC-derived optic vesicle (OV) organoids. Myoblasts cultured within the device exhibited high viability, successful differentiation, and the formation of aligned myotube bundles, outperforming conventional well-plate cultures. Additionally, iPSC-derived OV organoids-maintained viability, displayed neurite outgrowth, and sustained expression of the eye marker PAX6. These results demonstrate that the 3D-FlexTPU-MFD effectively supports cell growth, differentiation, and alignment, making it a promising platform for tissue modeling and OoC applications in future.  a2045-2322