02568nas a2200589   4500000000100000000000100001008004100002260001500043653001200058653001100070653001100081653002000092653001500112653000900127653003900136653001000175653001400185653003000199100001400229700002400243700002100267700002200288700001200310700002200322700002200344700001900366700002500385700002600410700002300436700002800459700001700487700001900504700001100523700002400534700001700558700002200575700001900597700002000616700002200636700002100658700002400679700001800703700002300721700001700744700002600761700002300787245009200810300001000902490000600912520104600918022001401964       2017                            d  c2017-03-2810aAnimals10aFemale10aHumans10aMenstrual Cycle10aMesothelin10aMice10aMicrofluidic Analytical Techniques10aOvary10aPregnancy10aTissue Culture Techniques1 aShuo Xiao1 aJonathan R. Coppeta1 aHunter B. Rogers1 aBrett C. Isenberg1 aJie Zhu1 aSusan A. Olalekan1 aKelly E. McKinnon1 aDanijela Dokic1 aAlexandra S. Rashedi1 aDaniel J. Haisenleder1 aSaurabh S. Malpani1 aChanel A. Arnold-Murray1 aKuanwei Chen1 aMingyang Jiang1 aLu Bai1 aCatherine T. Nguyen1 aJiyang Zhang1 aMonica M. Laronda1 aThomas J. Hope1 aKruti P. Maniar1 aMary Ellen Pavone1 aMichael J. Avram1 aElizabeth C. Sefton1 aSpiro Getsios1 aJoanna E. Burdette1 aJ. Julie Kim1 aJeffrey T. Borenstein1 aTeresa K. Woodruff00aA microfluidic culture model of the human reproductive tract and 28-day menstrual cycle  a145840 v83 aThe endocrine system dynamically controls tissue differentiation and homeostasis, but has not been studied using dynamic tissue culture paradigms. Here we show that a microfluidic system supports murine ovarian follicles to produce the human 28-day menstrual cycle hormone profile, which controls human female reproductive tract and peripheral tissue dynamics in single, dual and multiple unit microfluidic platforms (Solo-MFP, Duet-MFP and Quintet-MPF, respectively). These systems simulate the in vivo female reproductive tract and the endocrine loops between organ modules for the ovary, fallopian tube, uterus, cervix and liver, with a sustained circulating flow between all tissues. The reproductive tract tissues and peripheral organs integrated into a microfluidic platform, termed EVATAR, represents a powerful new in vitro tool that allows organ-organ integration of hormonal signalling as a phenocopy of menstrual cycle and pregnancy-like endocrine loops and has great potential to be used in drug discovery and toxicology studies.  a2041-1723