01838nas a2200241   4500000000100000000000100001008004100002260001500043653001800058653003500076100001300111700001800124700001900142700002500161700001700186700001900203700001900222245010600241856005500347300000800402520117200410022001401582       2025                            d  c2025-01-1610aCancer models10aExperimental models of disease1 aJihao Xu1 aMinh Duc Pham1 aVincenzo Corbo1 aMariano Ponz-Sarvise1 aTobiloba Oni1 aDaniel Öhlund1 aChang-Il Hwang00aAdvancing pancreatic cancer research and therapeutics: the transformative role of organoid technology  uhttps://www.nature.com/articles/s12276-024-01378-w  a1-93 aResearch on pancreatic cancer has transformed with the advent of organoid technology, providing a better platform that closely mimics cancer biology in vivo. This review highlights the critical advancements facilitated by pancreatic organoid models in understanding disease progression, evaluating therapeutic responses, and identifying biomarkers. These three-dimensional cultures enable the proper recapitulation of the cellular architecture and genetic makeup of the original tumors, providing insights into the complex molecular and cellular dynamics at various stages of pancreatic ductal adenocarcinoma (PDAC). We explore the applications of pancreatic organoids in dissecting the tumor microenvironment (TME); elucidating cancer progression, metastasis, and drug resistance mechanisms; and personalizing therapeutic strategies. By overcoming the limitations of traditional 2D cultures and animal models, the use of pancreatic organoids has significantly accelerated translational research, which is promising for improving diagnostic and therapeutic approaches in clinical settings, ultimately aiming to improve the outcomes of patients with pancreatic cancer.  a2092-6413