02545nas a2200397   4500000000100000000000100001008004100002260001500043653003200058653001200090653001200102653001700114653002300131100002100154700002300175700002600198700001600224700001700240700002300257700002200280700003000302700002400332700002400356700002000380700002400400700001900424700001800443700002500461700002300486700001700509245012600526856009300652490000700745520138100752022001402133       2023                            d  c2023-12-0610aFeasibility & Acceptability10aT cells10aTissues10aTuberculosis10apostmortem studies1 aGift Ahimbisibwe1 aMarjorie Nakibuule1 aMarvin Martin Ssejoba1 aDavid Oyamo1 aRose Mulwana1 aJosephine Nabulime1 aFebronius Babirye1 aMusana Abdusalaamu Kizito1 aHervé Monka Lekuya1 aAkello Suzan Adakun1 aDaisy Nalumansi1 aStella Muryasingura1 aRobert Lukande1 aAndrew Kyazze1 aJoseph Baruch Baluku1 aIrene Andia Biraro1 aStephen Cose00aFeasibility and acceptability of undertaking postmortem studies for tuberculosis medical research in a low income country  uhttps://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1264351/full0 v143 a<sec><title>Introduction</title><p>If we are to break new ground in difficult-to-treat or difficult-to-vaccinate diseases (such as HIV, malaria, or tuberculosis), we must have a better understanding of the immune system at the site of infection in humans. For tuberculosis (TB), the initial site of infection is the lungs, but obtaining lung tissues from subjects suffering from TB has been limited to bronchoalveolar lavage (BAL) or sputum sampling, or surgical resection of diseased lung tissue.</p></sec><sec><title>Methods</title><p>We examined the feasibility of undertaking a postmortem study for human tuberculosis research at Mulago National Referral Hospital in Kampala, Uganda.</p></sec><sec><title>Results</title><p>Postmortem studies give us an opportunity to compare TB-involved and -uninvolved sites, for both diseased and non-diseased individuals. We report good acceptability of the next-of-kin to consent for their relative’s tissue to be used for medical research; that postmortem and tissue processing can be undertaken within 8 hours following death; and that immune cells remain viable and functional up to 14 hours after death.</p></sec><sec><title>Discussion</title><p>Postmortem procedures remain a valuable and essential tool both to establish cause of death, and to advance our medical and scientific understanding of infectious diseases.</p></sec>  a1664-3224