02594nas a2200505   4500000000100000000000100001008004100002260001200043653001900055653002000074100002200094700002200116700001800138700002200156700002100178700002400199700002000223700002700243700002500270700001500295700001900310700002300329700001900352700002000371700002100391700001600412700001800428700001800446700001800464700002600482700002300508700002400531700001900555700002400574700002400598700002400622700002300646700002200669245007100691856005500762300001400817490000700831520123600838022001402074       2024                            d  c2024-0910aRNA sequencing10aTranscriptomics1 aMagda Marečková1 aLuz Garcia-Alonso1 aMarie Moullet1 aValentina Lorenzi1 aRobert Petryszak1 aCarmen Sancho-Serra1 aAgnes Oszlanczi1 aCecilia Icoresi Mazzeo1 aFrederick C. K. Wong1 aIva Kelava1 aSophie Hoffman1 aMichał Krassowski1 aKurtis Garbutt1 aKezia Gaitskell1 aSlaveya Yancheva1 aEe Von Woon1 aVictoria Male1 aIngrid Granne1 aKarin Hellner1 aKrishnaa T. Mahbubani1 aKourosh Saeb-Parsy1 aMohammad Lotfollahi1 aElena Prigmore1 aJennifer Southcombe1 aRebecca A. Dragovic1 aChristian M. Becker1 aKrina T. Zondervan1 aRoser Vento-Tormo00aAn integrated single-cell reference atlas of the human endometrium  uhttps://www.nature.com/articles/s41588-024-01873-w  a1925-19370 v563 aThe complex and dynamic cellular composition of the human endometrium remains poorly understood. Previous endometrial single-cell atlases profiled few donors and lacked consensus in defining cell types. We introduce the Human Endometrial Cell Atlas (HECA), a high-resolution single-cell reference atlas (313,527 cells) combining published and new endometrial single-cell transcriptomics datasets of 63 women with and without endometriosis. HECA assigns consensus and identifies previously unreported cell types, mapped in situ using spatial transcriptomics and validated using a new independent single-nuclei dataset (312,246 nuclei, 63 donors). In the functionalis, we identify intricate stromal–epithelial cell coordination via transforming growth factor beta (TGFβ) signaling. In the basalis, we define signaling between fibroblasts and an epithelial population expressing progenitor markers. Integration of HECA with large-scale endometriosis genome-wide association study data pinpoints decidualized stromal cells and macrophages as most likely dysregulated in endometriosis. The HECA is a valuable resource for studying endometrial physiology and disorders, and for guiding microphysiological in vitro systems development.  a1546-1718