02312nas a2200337   4500000000100000000000100001008004100002260000900043653001600052653002500068653001300093653001300106653001600119100001800135700002000153700001700173700001900190700003200209700002000241700001900261700002300280700001800303700001900321700002100340245012700361856006700488300001200555490000700567520138600574022001401960       2024                            d  c202410a3D printing10aextracellular matrix10aPolymers10ascaffold10aSkin models1 aFabien Girard1 aCaroline Lajoye1 aMarie Camman1 aNicolas Tissot1 aFlorence Berthelot Pedurand1 aBiranche Tandon1 aDenise Moedder1 aIevgenii Liashenko1 aSacha Salameh1 aPaul D. Dalton1 aMaïté Rielland00aFirst Advanced Bilayer Scaffolds for Tailored Skin Tissue Engineering Produced via Electrospinning and Melt Electrowriting  uhttps://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.202314757  a23147570 v343 aIn vitro skin models are validated methods for screening cosmetics and pharmaceuticals, but still have limitations. The bilayer poly(ε-caprolactone) scaffold/membrane model described here overcomes some of these deficits by integrating a solution electrospun (SES) membrane at the dermoepidermal interface and a melt electrowritten (MEW) scaffold that provides an optimal open-pore environment for the dermis. To the knowledge, this scaffold/membrane model is the only one capable of creating a properly differentiated, full thickness skin model with neosynthesized extracellular matrix (ECM) in only 18 days. Both the wavy and straight fiber scaffold designs create a well-organized dermis, but dermal collagen organization differs between designs. Adding cells and vitamin C to the scaffolds improves the mechanical properties to more closely mimic native human skin. These findings establish bicomponent scaffolds as a promising advancement for rapidly creating different skin models with varied properties. The versatility and adaptability of the described model can be used for studying how the biological and physical microenvironment impact skin, and testing dermo-cosmetics and pharmaceutical treatments on different ages of skin. Furthermore, it can be an excellent new tool for studying wound healing and development into its use as a graft or wound dressing is ongoing.  a1616-3028