02010nas a2200253   4500000000100000000000100001008004100002260001100043653002000054653002700074653001200101653001700113653001300130653002300143100001700166700001700183700001700200245007500217856004400292300000800336490000700344520139100351022001401742       2023                            d  c2023/110a3D bio-printing10aadditive manufacturing10abio-ink10abiomaterials10ascaffold10aTissue engineering1 aHumira Assad1 aArvina Assad1 aAshish Kumar00aRecent Developments in 3D Bio-Printing and Its Biomedical Applications  uhttps://www.mdpi.com/1999-4923/15/1/255  a2550 v153 aThe fast-developing field of 3D bio-printing has been extensively used to improve the usability and performance of scaffolds filled with cells. Over the last few decades, a variety of tissues and organs including skin, blood vessels, and hearts, etc., have all been produced in large quantities via 3D bio-printing. These tissues and organs are not only able to serve as building blocks for the ultimate goal of repair and regeneration, but they can also be utilized as in vitro models for pharmacokinetics, drug screening, and other purposes. To further 3D-printing uses in tissue engineering, research on novel, suitable biomaterials with quick cross-linking capabilities is a prerequisite. A wider variety of acceptable 3D-printed materials are still needed, as well as better printing resolution (particularly at the nanoscale range), speed, and biomaterial compatibility. The aim of this study is to provide expertise in the most prevalent and new biomaterials used in 3D bio-printing as well as an introduction to the associated approaches that are frequently considered by researchers. Furthermore, an effort has been made to convey the most pertinent implementations of 3D bio-printing processes, such as tissue regeneration, etc., by providing the most significant research together with a comprehensive list of material selection guidelines, constraints, and future prospects.  a1999-4923