01807nas a2200277   4500000000100000000000100001008004100002260001500043653002200058653002500080653001600105653002500121653002000146653003600166653000900202653001300211100002300224700001900247700001800266700002300284245008800307856011300395490000600508520100100514022001401515       2018                            d  c2018-10-3110a3D disease models10a3D tissue constructs10aBioprinting10aDermis and epidermis10aElectrospinning10aReconstructed human skin models10aSkin10ain vitro1 aMatthew J. Randall1 aAstrid Jüngel1 aMarkus Rimann1 aKarin Wuertz-Kozak00aAdvances in the Biofabrication of 3D Skin in vitro: Healthy and Pathological Models  uhttps://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2018.00154/full0 v63 a<p>The relevance for <italic>in vitro</italic> three-dimensional (3D) tissue culture of skin has been present for almost a century. From using skin biopsies in organ culture, to vascularized organotypic full-thickness reconstructed human skin equivalents, <italic>in vitro</italic> tissue regeneration of 3D skin has reached a golden era. However, the reconstruction of 3D skin still has room to grow and develop. The need for reproducible methodology, physiological structures and tissue architecture, and perfusable vasculature are only recently becoming a reality, though the addition of more complex structures such as glands and tactile corpuscles require advanced technologies. In this review, we will discuss the current methodology for biofabrication of 3D skin models and highlight the advantages and disadvantages of the existing systems as well as emphasize how new techniques can aid in the production of a truly physiologically relevant skin construct for preclinical innovation.</p>  a2296-4185