01695nas a2200217   4500000000100000000000100001008004100002260001500043653002200058653002300080653002200103100001900125700001700144700001900161245007300180856004700253300001200300490000700312520114400319022001401463       2023                            d  c2023-07-0110aAtopic dermatitis10aIn vitro 3D models10aSkin inflammation1 aHye-Jeong Jang1 aJung Bok Lee1 aJeong-Kee Yoon00aAdvanced In Vitro Three-Dimensional Skin Models of Atopic Dermatitis  uhttps://doi.org/10.1007/s13770-023-00532-1  a539-5520 v203 aAtopic dermatitis (AD) is one of the most prevalent inflammatory skin diseases that is characterized by eczematous rashes, intense itching, dry skin, and sensitive skin. Although AD significantly impacts the quality of life and the number of patients keeps increasing, its pathological mechanism is still unknown because of its complexity. The importance of developing new in vitro three-dimensional (3D) models has been underlined in order to understand the mechanisms for the development of therapeutics since the limitations of 2D models or animal models have been repeatedly reported. Thus, the new in vitro AD models should not only be created in 3D structure, but also reflect the pathological characteristics of AD, which are known to be associated with Th2-mediated inflammatory responses, epidermal barrier disruption, increased dermal T-cell infiltration, filaggrin down-regulation, or microbial imbalance. In this review, we introduce various types of in vitro skin models including 3D culture methods, skin-on-a-chips, and skin organoids, as well as their applications to AD modeling for drug screening and mechanistic studies.  a2212-5469