02374nas a2200229   4500000000100000000000100001008004100002260001200043653002200055653001600077100002000093700001800113700002300131700001800154700002100172245010300193856005400296300001200350490000700362520176100369022001402130       2020                            d  c2020-0210aBiological models10aBiomimetics1 aKambez H. Benam1 aRichard Novak1 aThomas C. Ferrante1 aYoungjae Choe1 aDonald E. Ingber00aBiomimetic smoking robot for in vitro inhalation exposure compatible with microfluidic organ chips  uhttps://www.nature.com/articles/s41596-019-0230-y  a183-2060 v153 aExposure of lung tissues to cigarette smoke is a major cause of human disease and death worldwide. Unfortunately, adequate model systems that can reliably recapitulate disease biogenesis in vitro, including exposure of the human lung airway to fresh whole cigarette smoke (WCS) under physiological breathing airflow, are lacking. This protocol extension builds upon, and can be used with, our earlier protocol for microfabrication of human organs-on-chips. Here, we describe the engineering, assembly and operation of a microfluidically coupled, multi-compartment platform that bidirectionally ‘breathes’ WCS through microchannels of a human lung small airway microfluidic culture device, mimicking how lung cells may experience smoke in vivo. Several WCS-exposure systems have been developed, but they introduce smoke directly from above the cell cultures, rather than tangentially as naturally occurs in the lung due to lateral airflow. We detail the development of an organ chip–compatible microrespirator and a smoke machine to simulate breathing behavior and smoking topography parameters such as puff time, inter-puff interval and puffs per cigarette. Detailed design files, assembly instructions and control software are provided. This novel platform can be fabricated and assembled in days and can be used repeatedly. Moderate to advanced engineering and programming skills are required to successfully implement this protocol. When coupled with the small airway chip, this protocol can enable prediction of patient-specific biological responses in a matched-comparative manner. We also demonstrate how to adapt the protocol to expose living ciliated airway epithelial cells to smoke generated by electronic cigarettes (e-cigarettes) on-chip.  a1750-2799