02059nas a2200337   4500000000100000000000100001008004100002260001500043653002800058653001500086653002600101653002300127100002000150700002200170700003200192700001600224700002900240700003400269700001800303700002300321700002500344700002000369700001900389700001900408245007000427856005500497300000900552490000600561520114000567022001401707       2017                            d  c2017-11-2410aAntimicrobial responses10aChemokines10aMicrobial communities10aTissue engineering1 aLayla J. Barkal1 aClare L. Procknow1 aYasmín R. Álvarez-García1 aMengyao Niu1 aJosé A. Jiménez-Torres1 aRebecca A. Brockman-Schneider1 aJames E. Gern1 aLoren C. Denlinger1 aAshleigh B. Theberge1 aNancy P. Keller1 aErwin Berthier1 aDavid J. Beebe00aMicrobial volatile communication in human organotypic lung models  uhttps://www.nature.com/articles/s41467-017-01985-4  a17700 v83 aWe inhale respiratory pathogens continuously, and the subsequent signaling events between host and microbe are complex, ultimately resulting in clearance of the microbe, stable colonization of the host, or active disease. Traditional in vitro methods are ill-equipped to study these critical events in the context of the lung microenvironment. Here we introduce a microscale organotypic model of the human bronchiole for studying pulmonary infection. By leveraging microscale techniques, the model is designed to approximate the structure of the human bronchiole, containing airway, vascular, and extracellular matrix compartments. To complement direct infection of the organotypic bronchiole, we present a clickable extension that facilitates volatile compound communication between microbial populations and the host model. Using Aspergillus fumigatus, a respiratory pathogen, we characterize the inflammatory response of the organotypic bronchiole to infection. Finally, we demonstrate multikingdom, volatile-mediated communication between the organotypic bronchiole and cultures of Aspergillus fumigatus and Pseudomonas aeruginosa.  a2041-1723