03514nas a2200901   4500000000100000000000100001008004100002260001200043653001000055653001000065653001200075653001000087653001800097653002200115653002700137653002100164653001100185653001000196653001100206653001100217653001500228653001600243653000900259653002300268653001800291653001900309653002400328653002900352653001700381100002000398700002300418700001800441700001800459700001400477700001900491700002700510700002100537700002100558700001400579700001900593700001800612700001300630700002200643700002100665700002000686700001800706700001900724700002400743700002200767700001600789700001800805700002900823700002100852700002100873700002200894700002300916700002100939700001600960700002200976700002000998700002301018700002101041700001601062700002001078700001801098700002601116700002301142700002201165700002401187700002101211700001901232700002301251245006901274300001201343490000801355520123501363022001402598       2021                            d  c2021-0910aAdult10aaging10aAnimals10aChild10aCrohn Disease10aDatasets as Topic10aEnteric Nervous System10aEpithelial Cells10aFemale10aFetus10aHealth10aHumans10aIntestines10aLymph Nodes10aMice10aMice, Inbred C57BL10aOrganogenesis10aReceptors, IgG10aSignal Transduction10aSpatio-Temporal Analysis10aTime Factors1 aRasa Elmentaite1 aNatsuhiko Kumasaka1 aKenny Roberts1 aAaron Fleming1 aEmma Dann1 aHamish W. King1 aVitalii Kleshchevnikov1 aMonika Dabrowska1 aSophie Pritchard1 aLiam Bolt1 aSara F. Vieira1 aLira Mamanova1 aNi Huang1 aFrancesca Perrone1 aIssac Goh Kai'En1 aSteven N. Lisgo1 aMatilda Katan1 aSteven Leonard1 aThomas R. W. Oliver1 aC. Elizabeth Hook1 aKomal Nayak1 aLia S. Campos1 aCecilia Domínguez Conde1 aEmily Stephenson1 aJustin Engelbert1 aRachel A. Botting1 aKrzysztof Polanski1 aStijn van Dongen1 aMinal Patel1 aMichael D. Morgan1 aJohn C. Marioni1 aOmer Ali Bayraktar1 aKerstin B. Meyer1 aXiaoling He1 aRoger A. Barker1 aHolm H. Uhlig1 aKrishnaa T. Mahbubani1 aKourosh Saeb-Parsy1 aMatthias Zilbauer1 aMenna R. Clatworthy1 aMuzlifah Haniffa1 aKylie R. James1 aSarah A. Teichmann00aCells of the human intestinal tract mapped across space and time  a250-2550 v5973 aThe cellular landscape of the human intestinal tract is dynamic throughout life, developing in utero and changing in response to functional requirements and environmental exposures. Here, to comprehensively map cell lineages, we use single-cell RNA sequencing and antigen receptor analysis of almost half a million cells from up to 5 anatomical regions in the developing and up to 11 distinct anatomical regions in the healthy paediatric and adult human gut. This reveals the existence of transcriptionally distinct BEST4 epithelial cells throughout the human intestinal tract. Furthermore, we implicate IgG sensing as a function of intestinal tuft cells. We describe neural cell populations in the developing enteric nervous system, and predict cell-type-specific expression of genes associated with Hirschsprung's disease. Finally, using a systems approach, we identify key cell players that drive the formation of secondary lymphoid tissue in early human development. We show that these programs are adopted in inflammatory bowel disease to recruit and retain immune cells at the site of inflammation. This catalogue of intestinal cells will provide new insights into cellular programs in development, homeostasis and disease.  a1476-4687