02031nas a2200445   4500000000100000000000100001008004100002260001500043653002100058653001400079653001000093653003000103653004600133653001100179653001200190653002500202653002600227100001500253700001900268700002100287700002100308700001300329700001700342700002100359700002500380700002500405700001400430700002000444700001600464700001600480700002200496700001700518700001800535700001700553245004800570300001300618490000800631520093200639022001401571       2020                            d  c2020-11-1310aAtlases as Topic10aChromatin10aFetus10aGene Expression Profiling10aGene Expression Regulation, Developmental10aHumans10aNeurons10aSingle-Cell Analysis10aTranscription Factors1 aJunyue Cao1 aDiana R. O'Day1 aHannah A. Pliner1 aPaul D. Kingsley1 aMei Deng1 aRiza M. Daza1 aMichael A. Zager1 aKimberly A. Aldinger1 aRonnie Blecher-Gonen1 aFan Zhang1 aMalte Spielmann1 aJames Palis1 aDan Doherty1 aFrank J. Steemers1 aIan A. Glass1 aCole Trapnell1 aJay Shendure00aA human cell atlas of fetal gene expression  aeaba77210 v3703 aThe gene expression program underlying the specification of human cell types is of fundamental interest. We generated human cell atlases of gene expression and chromatin accessibility in fetal tissues. For gene expression, we applied three-level combinatorial indexing to >110 samples representing 15 organs, ultimately profiling ~4 million single cells. We leveraged the literature and other atlases to identify and annotate hundreds of cell types and subtypes, both within and across tissues. Our analyses focused on organ-specific specializations of broadly distributed cell types (such as blood, endothelial, and epithelial), sites of fetal erythropoiesis (which notably included the adrenal gland), and integration with mouse developmental atlases (such as conserved specification of blood cells). These data represent a rich resource for the exploration of in vivo human gene expression in diverse tissues and cell types.  a1095-9203