02478nas a2200637   4500000000100000008004100001260001500042100002300057700002200080700001700102700001900119700002000138700003000158700002500188700001700213700001800230700002400248700001900272700002300291700002000314700002800334700001400362700002200376700002100398700001000419700002400429700002500453700001600478700001900494700002600513700002000539700001800559700002300577700002800600700001500628700002000643700002500663700001900688700001800707700001600725700001600741700001700757700001700774700001400791700002100805700001500826700002100841700002800862700001500890245010100905856007201006300001201078490000701090520072901097022001401826       2024                            d  c2024-06-111 aSoraya O. Sandoval1 aGerarda Cappuccio1 aKarina Kruth1 aSivan Osenberg1 aSaleh M. Khalil1 aNatasha M. Méndez-Albelo1 aKrishnan Padmanabhan1 aDaifeng Wang1 aMark J. Niciu1 aAnita Bhattacharyya1 aJason L. Stein1 aAndré M. M. Sousa1 aElisa A. Waxman1 aElizabeth D. Buttermore1 aDosh Whye1 aCarissa L. Sirois1 aStewart Anderson1 aAnita1 aAnita Bhattacharyya1 aElizabeth Buttermore1 aQiang Chang1 aDeborah French1 aKazue Hashimoto-Torii1 aHarley Kornblum1 aKristin Kroll1 aHerbert M. Lachman1 aMirjana Maletic-Savatic1 aMark Niciu1 aBennett Novitch1 aKrishnan Padmanabhan1 aChris Proschel1 aMustafa Sahin1 aAndre Sousa1 aJason Stein1 aDaifeng Wang1 aElisa Waxman1 aDosh Whye1 aAislinn Williams1 aXinyu Zhao1 aAislinn Williams1 aMirjana Maletic-Savatic1 aXinyu Zhao00aRigor and reproducibility in human brain organoid research: Where we are and where we need to go  uhttps://www.sciencedirect.com/science/article/pii/S2213671124001140  a796-8160 v193 aHuman brain organoid models have emerged as a promising tool for studying human brain development and function. These models preserve human genetics and recapitulate some aspects of human brain development, while facilitating manipulation in an in vitro setting. Despite their potential to transform biology and medicine, concerns persist about their fidelity. To fully harness their potential, it is imperative to establish reliable analytic methods, ensuring rigor and reproducibility. Here, we review current analytical platforms used to characterize human forebrain cortical organoids, highlight challenges, and propose recommendations for future studies to achieve greater precision and uniformity across laboratories.  a2213-6711