01101nas a2200313   4500000000100000000000100001008004100002260001200043653002400055653001500079653001100094100001800105700002100123700001600144700001700160700001800177700001800195700001800213700002000231700001600251700001900267700002100286245004400307856004700351300001200398490000700410520035600417022001400773       2016                            d  c2016-1010aAntibody generation10aImmunology10apolicy1 aMathias Uhlen1 aAnita Bandrowski1 aSteven Carr1 aAled Edwards1 aJan Ellenberg1 aEmma Lundberg1 aDavid L. Rimm1 aHenry Rodriguez1 aTara Hiltke1 aMichael Snyder1 aTadashi Yamamoto00aA proposal for validation of antibodies  uhttps://www.nature.com/articles/nmeth.3995  a823-8270 v133 aWe convened an ad hoc International Working Group for Antibody Validation in order to formulate the best approaches for validating antibodies used in common research applications and to provide guidelines that ensure antibody reproducibility. We recommend five conceptual 'pillars' for antibody validation to be used in an application-specific manner.  a1548-7105