02800nas a2200325   4500000000100000000000100001008004100002260000900043653000800052653001100060653005600071653001500127653002300142653001300165653002000178100002100198700002700219700002500246700001900271700001500290700002200305700002600327700001800353245010500371856006700476300001200543490000700555520189800562022001402460       2022                            d  c202210aFBS10aGIVIMP10aGuidance Document on Good In vitro Method Practices10aantibodies10afetal bovine serum10ain vitro10areproducibility1 aManuela Cassotta1 aJoanna Julia Bartnicka1 aFrancesca Pistollato1 aSurat Parvatam1 aTilo Weber1 aVito D'Alessandro1 aLuisa Ferreira Bastos1 aSandra Coecke00aA worldwide survey on the use of animal-derived materials and reagents in scientific experimentation  uhttps://onlinelibrary.wiley.com/doi/abs/10.1002/elsc.202100167  a564-5830 v223 aThe use of cell and tissue-based methods in basic, applied and regulatory science has been increasing exponentially. Animal-derived components, including serum, coating materials, growth factors and antibodies are routinely used in cell/tissue cultures and in general laboratory practices. In addition to ethical issues, the use and production of animal-derived materials and reagents raises many scientific concerns, generally associated with presence of undefined components and batch-to-batch variability, which may compromise experimental reproducibility. On the other hand, non-animal materials and reagents, such as human cells, alternatives to animal sera or non-animal recombinant antibodies, are becoming increasingly available, and their use is encouraged by the EU Directive 2010/63 and the Guidance Document on Good In vitro Method Practices (GIVIMP), published by the Organization for Economic Cooperation and Development (OECD). In an effort to map the current state of use of animal-derived reagents across different sectors and to identify the obstacles possibly hampering the implementation of non-animal derived alternatives, a global online survey addressed to scientists working on in vivo, in vitro, in silico methods, in academia as well as pharmaceutical or cosmetic companies, was conducted with the goal to understand: 1) the most commonly used animal-derived materials and reagents, 2) the main issues associated with the production and use of animal-derived materials and reagents, 3) the current level of knowledge on available non-animal alternative materials and reagents, and 4) what educational and information sources could be most useful or impactful to disseminate knowledge on non-animal alternatives. This paper provides an overview of the survey replies and discusses possible proposals to increase awareness, acceptance and use of non-animal ingredients.  a1618-2863