01923nas a2200325   4500000000100000000000100001008004100002260001500043653001700058653002000075653001300095653004400108100001700152700001800169700001700187700001700204700002300221700002500244700002400269700002200293700002900315700002100344700002700365245008200392856005500474300000900529490000700538520103800545022001401583       2019                            d  c2019-07-1610aCell biology10aCell signalling10aDiseases10aEndocrine system and metabolic diseases1 aKyu Shik Mun1 aKavisha Arora1 aYunjie Huang1 aFanmuyi Yang1 aSunitha Yarlagadda1 aYashaswini Ramananda1 aMaisam Abu-El-Haija1 aJoseph J. Palermo1 aBalamurugan N. Appakalai1 aJaimie D. Nathan1 aAnjaparavanda P. Naren00aPatient-derived pancreas-on-a-chip to model cystic fibrosis-related disorders  uhttps://www.nature.com/articles/s41467-019-11178-w  a31240 v103 aCystic fibrosis (CF) is a genetic disorder caused by defective CF Transmembrane Conductance Regulator (CFTR) function. Insulin producing pancreatic islets are located in close proximity to the pancreatic duct and there is a possibility of impaired cell-cell signaling between pancreatic ductal epithelial cells (PDECs) and islet cells as causative in CF. To study this possibility, we present an in vitro co-culturing system, pancreas-on-a-chip. Furthermore, we present an efficient method to micro dissect patient-derived human pancreatic ducts from pancreatic remnant cell pellets, followed by the isolation of PDECs. Here we show that defective CFTR function in PDECs directly reduced insulin secretion in islet cells significantly. This uniquely developed pancreatic function monitoring tool will help to study CF-related disorders in vitro, as a system to monitor cell-cell functional interaction of PDECs and pancreatic islets, characterize appropriate therapeutic measures and further our understanding of pancreatic function.  a2041-1723