01850nas a2200265   4500000000100000000000100001008004100002260001500043653002100058653001800079653002000097653002000117653002200137100001700159700001500176700002100191700001900212700001600231245007400247856004600321300000700367490000700374520118900381022001401570       2019                            d  c2019-11-0410aEx vivo analysis10aFraction chip10aIslet-on-a-chip10aMicromesh sheet10apancreatic islets1 aTakeshi Hori1 aKei Yamane1 aTakayuki Anazawa1 aOsamu Kurosawa1 aHiroo Iwata00aCompact fluidic system for functional assessment of pancreatic islets  uhttps://doi.org/10.1007/s10544-019-0443-4  a910 v213 aTransplantation of pancreatic islets is becoming a promising therapy for people with type I diabetes. In this study, we present a compact fluidic system that enables assessment of islet functionality ex vivo for efficient islet transplantation. The fluidic system includes a micromesh sheet-embedded chip. Islets can be loaded easily on the micromesh sheet and observed clearly by microscopy. Islets on the mesh sheet mainly remained in place during perfusion and did not get damaged by hydraulic pressure because of high porosity of the micromesh sheet. The fluidic system was assembled with a sample fraction chip of polydimethylsiloxane. The chip includes a channel and columns, both having surfaces that were super-hydrophilized so that solutions could flow smoothly within the chip by gravity. Using mouse pancreatic islets, a dynamic glucose-stimulated insulin secretion test was performed to examine the performance of the fluidic system. The system successfully analyzed levels and patterns of insulin secretion upon exposure of the islets to low- and high-glucose solutions in turns, thus demonstrating its capacity to assess islet functions more easily and cost-effectively.  a1572-8781