01955nas a2200289   4500000000100000000000100001008004100002260001500043653002100058653002500079653001400104653002200118100002500140700001600165700001700181700001600198700001800214700001800232700001800250700002100268700002600289700001900315245010300334300001300437520120100450022001401651       2023                            d  c2023-10-2210across-talk study10aglomerulus-on-a-chip10amesangium10atri-culture model1 aRamin Pajoumshariati1 aLorna Ewart1 aVille Kujala1 aRaymond Luc1 aSamantha Peel1 aAdam Corrigan1 aHeather Weber1 aBramasta Nugraha1 aPernille B. L. Hansen1 aJulie Williams00aPhysiological Replication of the Human Glomerulus Using a Triple Culture Microphysiological System  ae23031313 aThe function of the glomerulus depends on the complex cell-cell/matrix interactions and replication of this in vitro would aid biological understanding in both health and disease. Previous models do not fully reflect all cell types and interactions present as they overlook mesangial cells within their 3D matrix. Herein, the development of a microphysiological system that contains all resident renal cell types in an anatomically relevant manner is presented. A detailed transcriptomic analysis of the contributing biology of each cell type, as well as functionally appropriate albumin retention in the system, is demonstrated. The important role of mesangial cells is shown in promoting the health and maturity of the other cell types. Additionally, a comparison of the incremental advances that each individual cell type brings to the phenotype of the others demonstrates that glomerular cells in simple 2D culture exhibit a state more reflective of the dysfunction observed in human disease than previously recognized. This in vitro model will expand the capability to investigate glomerular biology in a more translatable manner by the inclusion of the important mesangial cell compartment.  a2198-3844