01788nas a2200301   4500000000100000000000100001008004100002260001500043653001700058653001500075653001800090653002100108653002000129653001500149653001700164653001500181100002100196700001800217700001500235700001700250700001600267245008600283856004700369300001400416490000700430520103500437022001401472       2021                            d  c2021-12-0110aAngiogenesis10aCD4 T cell10adisease model10aendothelial cell10ain vitro models10aMacrophage10amicrofluidic10aNeutrophil1 aJustin Silberman1 aAakanksha Jha1 aHolly Ryan1 aTalia Abbate1 aErika Moore00aModeled vascular microenvironments: immune-endothelial cell interactions in vitro  uhttps://doi.org/10.1007/s13346-021-00970-1  a2482-24950 v113 aThe advancement of in vitro techniques enables a better understanding of biological processes and improves drug screening platforms. In vitro studies allow for enhanced observation of cell behavior, control over the mimicked microenvironment, and the ability to use human cells. In particular, advances in vascular microenvironment recapitulation are of interest given vasculature influence in cardiovascular vascular diseases and cancer. These investigate alterations in endothelial cell behavior and immune cell interactions with endothelial cells. Specific immune cells such as monocytes, macrophages, neutrophils, and T cells influence endothelial cell behavior by promoting or inhibiting vasculogenesis through cell-cell interaction or soluble signaling. Results from these studies showcase cell behavior in vascular diseases and in the context of tumor metastasis. In this review, we discuss examples of in vitro studies modeling immune cell-endothelial cell interactions to present methods and recent findings in the field.  a2190-3948